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首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >EVALUATION OF THE METROLOGICAL PARAMETERS OF METHODS FOR QUANTITATIVE DETERMINATION OF ACTIVE MICROBIAL STRAINS OF GENERA PSEUDOMONAS AND BACILLUS IN THE AIR OF BIOTECHNOLOGICAL PRODUCTION ZONE
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EVALUATION OF THE METROLOGICAL PARAMETERS OF METHODS FOR QUANTITATIVE DETERMINATION OF ACTIVE MICROBIAL STRAINS OF GENERA PSEUDOMONAS AND BACILLUS IN THE AIR OF BIOTECHNOLOGICAL PRODUCTION ZONE

机译：定量测定生物技术生产区空气中假单胞菌和芽孢杆菌活性微生物菌株方法的气象参数评估

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Provision of safety and sanitary-hygienic control of biotechnological processes poses a special medical-biological challenge requiring elaboration and metrological assessment of quantitative methods, which determine concentrations of microorganisms-producers in air of manufacturing zone in compliance with the criteria of the International Organization for Standardization. The results of experimental modeling of microbial sprays comprising bacteria of genera Bacillus and Pseudomonas in air of the production zone as the risk factor affecting health of bioindustry employees have enabled to calculate metrological parameters of methods to control microorganisms-constituents of monocomponent and bicomponent biological preparations. Such metrological parameters (standard deviation of repeatability, standard deviation of intermediate precision, expanded uncertainty) match the requirements set to the measurement methods at the accepted confidential probability level p=95 % and ensure proper control of biotechnological processes.
机译：提供生物技术过程的安全和卫生控制带来了特殊的医学-生物学挑战，需要对定量方法进行详细的设计和计量评估，以根据国际标准化组织的标准确定生产区空气中微生物产生者的浓度。对产区空气中包含芽孢杆菌属和假单胞菌属细菌作为影响生物工业员工健康的危险因素的微生物喷雾剂进行实验建模的结果，使得能够计算控制单组分和双组分生物制剂中微生物成分的方法的计量参数。这样的计量参数（可重复性的标准偏差，中间精度的标准偏差，不确定性的扩展）在公认的机密概率水平p = 95％时符合测量方法的要求，并确保对生物技术过程的适当控制。

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