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首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >POTENTIAL EFFECT OF ANTAGONISTIC BACTERIA IN THE MANAGEMENT OF OLIVE KNOT DISEASE CAUSED BY PSEUDOMONAS SAVASTANOI PV. SAVASTANOI
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POTENTIAL EFFECT OF ANTAGONISTIC BACTERIA IN THE MANAGEMENT OF OLIVE KNOT DISEASE CAUSED BY PSEUDOMONAS SAVASTANOI PV. SAVASTANOI

机译:假单胞菌PV引起的细菌性结节性疾病的拮抗作用。萨瓦斯塔诺伊

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Olive knot disease caused by Pseudomonas savastanoi pv. savastanoi (PSS) is considered as the main bacterial disease of olive tree in Morocco. However, sole prophylactic measures are available. The main aim of this work was to evaluate the effects of eighteen antagonistic bacteria, as an alternative approach, to control in vitro and in planta the pathogens strains previously isolated from olive trees in Morocco. In vitro experiments revealed that many bacterial strains such as Rahnella aquatilis, Enterobacter ludwiqii, Paenibacillus brasiliensis, and Bacillus subtilis selected from collection of Regional Center of Agronomic Research, were efficient on solid media against pathogen strains. Their inhibition zones ranged from 8 to 23 mm with a higher inhibition rates found by B. subtilis strains. Enzymatic activities of antagonistic strains were assessed (cellulase, protease and amylase) as well as some plant growth promotion traits (phosphorus solubilisation and indole acetic acid production) for performance screening. B. subtilis 2515-1 was selected for pots experiment using olive trees (cv. Arbequina). This bacterium significantly reduced number and weight of knots against two pathogens strains (PSS 2064-8 and PSS 2102-4). The most reduction rates of weight and knot number were respectively 95.60% and 50%. This study suggested an alternative approach to assess the efficiency of selected bacterium in field conditions.
机译:萨瓦氏假单胞菌引起的橄榄结病。 savastanoi(PSS)被认为是摩洛哥橄榄树的主要细菌病。但是,可以采取唯一的预防措施。这项工作的主要目的是评估十八种拮抗细菌的作用,作为一种替代方法,可以控制体外和植物体内先前从摩洛哥橄榄树中分离出的病原体。体外实验表明,从农学研究中心区域选集中选出的许多细菌菌株,如水生拉氏菌,卢氏小肠杆菌,巴西芽孢杆菌和枯草芽孢杆菌,对固体培养基均能有效抵抗病原菌。它们的抑制区范围为8至23 mm,枯草芽孢杆菌菌株发现的抑制率更高。评估拮抗菌株的酶活性(纤维素酶,蛋白酶和淀粉酶)以及一些植物生长促进性状(磷增溶和吲哚乙酸生产)以进行性能筛选。选择枯草芽孢杆菌2515-1进行橄榄树盆栽试验(cv。Arbequina)。该细菌显着减少了对两种病原体菌株(PSS 2064-8和PSS 2102-4)的打结数量和重量。减重率最高,分别为95.60%和50%。这项研究提出了另一种方法来评估田间条件下所选细菌的效率。

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