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首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >IN VITRO ASSESSMENT OF IRON EFFECT ON THE SPERMATOZOA MOTILITY PARAMETERS
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IN VITRO ASSESSMENT OF IRON EFFECT ON THE SPERMATOZOA MOTILITY PARAMETERS

机译：铁对精子活动性参数的体外评估

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Iron (Fe) is an essential element but on the other hand it could induce changes in reproductive system. The general objective of this in vitro study was at first to examine dose- and time-dependent effects of iron (ferrous sulphate heptahydrate - FeSO4.7H2O) on the spermatozoa motility parameters, secondly expand the knowledge concerning direct action of this metal on the fertilization potential of the spermatozoa. The motility analysis was determined after exposure to concentrations of 3.9; 7.8; 15.6; 31.2; 62.5; 125; 250; 500; 1000 μmol.dm-3 of FeSO4.7H2O using the Sperm VisionTM CASA (Computer Assisted Semen Analyzer) system during different time periods (Time 0 h, 2 h and 24 h). The highest percentage of motile spermatozoa was detected in the control group (95.41±1.32%) (Time 0 h). After 2 h of cultivation with ferrous sulphate heptahydrate the motility spermatozoa significantly (P<0.001) increased at the concentrations ≤ 125 μmol.dm-3. The experimental administration at the doses ≥ 125 μmol.dm-3 FeSO4.7H2O inhibited the overall percentage of spermatozoa motility during Time 24 h. The identical spermatozoa motility was detected also for the percentage of progressive motile spermatozoa during all time periods. Detailed evaluation of spermatozoa distance average (DAP) and velocity average (VAP) path as well as amplitude of lateral head displacement (ALH) revealed decrease in groups with concentrations ≥ 125 μmol.dm-3 FeSO4.7H2O in comparison with the control group during the long-term cultivation. Based on these results, we can conclude that the iron at the low concentrations maintains the spermatozoa motility parameters. This essential element has probably direct action on the fertilization potential of the spermatozoa, what could be used in assisted reproductive technologies.
机译：铁（Fe）是必不可少的元素，但另一方面，它可能导致生殖系统发生变化。这项体外研究的总体目标是，首先研究铁（七水合硫酸亚铁-FeSO4.7H2O）对精子活力参数的剂量和时间依赖性影响，其次扩大关于这种金属对受精的直接作用的知识。精子的潜力。在暴露于3.9的浓度后确定运动分析; 7.8; 15.6; 31.2; 62.5; 125; 250; 500;使用Sperm VisionTM CASA（计算机辅助精液分析仪）系统在不同时间段（0小时，2小时和24小时）中提取1000μmol.dm-3的FeSO4.7H2O。在对照组中，活动精子的百分比最高（95.41±1.32％）（时间为0 h）。用七水合硫酸亚铁培养2小时后，在浓度≤125μmol.dm-3时，精子的活力显着提高（P <0.001）。 ≥125μmol.dm-3FeSO4.7H2O的实验性给药在24小时内抑制了精子活力的总体百分比。在所有时间段内，对于进行性运动精子的百分比也检测到相同的精子运动。详细评估精子距离平均（DAP）和速度平均（VAP）路径以及侧向头部移位（ALH）的幅度后发现，与对照组相比，浓度≥125μmol.dm-3FeSO4.7H2O的组的精子减少了。长期种植。根据这些结果，我们可以得出结论，低浓度的铁保持了精子活力参数。该基本元素可能对精子的受精潜力具有直接作用，可将其用于辅助生殖技术。

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