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首页> 外文期刊>Journal of Microbiology, Biotechnology and Food Sciences >PROPAGATION OF CLEOME SPINOSA JACQ. THROUGH TISSUE CULTURE
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PROPAGATION OF CLEOME SPINOSA JACQ. THROUGH TISSUE CULTURE

机译：CLEOME SPINOSA JACQ的繁殖。通过组织文化

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摘要

The tissue culture and rapid propagation of Cleome spinosa Jacq. was explored by investigating the effects of different plant growth regulators on callus induction, bud differentiation, and root formation of three types of explants. The results showed that hypocotyls and stem segments regenerated buds directly on growth regulator-free Murashige and Skoog (MS)medium. The highest callus induction rates of hypocotyls, stem segments, and leaves reached 100% and were obtained on the culture medium of MS + (1.0 to 2.0) mg/L kinetin (KT) + 0.02 mg/L α-naphthalene acetic acid (NAA), on which the leaves produced the best quality of calluses. The leaf-derived calluses were subcultured on MS + 0.5 mg/L KT +0.5 mg/L 6-benzylaminopurine (BAP) and achieved the highest differentiation rate of 100%, producing an average of 7.5 buds per explant. Inoculation with MS + 0.5 mg/L indole-3-butyric acid (IBA) resulted in the production of a number of thick roots by 66.7% of the regenerated buds. After transplanting, plantlets with more roots survived easily and grew well.
机译：Cleome spinosa Jacq的组织培养和快速繁殖。通过研究不同的植物生长调节剂对三种类型外植体的愈伤组织诱导，芽分化和根形成的影响，探索了该基因。结果表明，下胚轴和茎段直接在无生长调节剂的Murashige和Skoog（MS）培养基上再生芽。在MS +（1.0至2.0）mg / L激动素（KT）+ 0.02 mg / Lα-萘乙酸（NAA）的培养基上获得最高的胚轴，茎节和叶的愈伤组织诱导率达到100％ ），叶子上的老茧质量最好。将叶片衍生的愈伤组织在MS + 0.5 mg / L KT +0.5 mg / L 6-苄基氨基嘌呤（BAP）上传代培养，达到最高分化率100％，平均每个外植体产生7.5个芽。 MS + 0.5 mg / L吲哚-3-丁酸（IBA）接种后，再生芽的66.7％产生了许多粗根。移栽后，具有更多根的小植株容易存活并生长良好。

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