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首页> 外文期刊>Journal of Molecular Genetics >Studies and Characterization of Bacterial Spot Pathogen of Tomato Xanthomonas campestris PV Vesicatoria
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Studies and Characterization of Bacterial Spot Pathogen of Tomato Xanthomonas campestris PV Vesicatoria

机译：番茄Xanthomonas campestris PV Vesicatoria细菌斑病病原菌的研究与鉴定

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摘要

Investigation was conducted in the laboratory to characterize, identify and determine the strains of bacteria responsible for bacterial spot disease of tomato in humid tropics of South Eastern Nigeria. The bacteria were isolated from infected tomato plant; leaf, fruit and seed and subjected to some standard bacteriological tests. The results of the cultural, physiological and biochemical analyses obtained showed that the bacteria isolated from bacterial spot lesions of tomato fruits, leaves and seed were gram negative, yellow aerobic rod shaped bacteria with a polar flagella. The bacteria colonies exhibited strong starch hydrolysis, metabolized glucose and produced acid from arabinose, sucrose and cellobiose but not from ducitol or sorbitol. Also nitrite was not reduced to nitrite based on bacteriological characteristics, the bacteria strains were identified as Xanthomonas campestris pv. vesicatoria (ex Doidge). The pathogens were found to be influenced by temperature with optimum temperature of 30-32°C but little or no growth at 20 and 45°C.
机译：在实验室进行了调查，以表征，鉴定和确定造成尼日利亚东南部热带湿润地区番茄细菌斑病的细菌菌株。从感染的番茄植物中分离出细菌;叶子，果实和种子，并经过一些标准的细菌学测试。所获得的文化，生理和生化分析结果表明，从番茄果实，叶片和种子的细菌斑点损伤中分离出的细菌为革兰氏阴性黄色需氧杆状细菌，带有极鞭毛。细菌菌落表现出强烈的淀粉水解作用，代谢了葡萄糖，并由阿拉伯糖，蔗糖和纤维二糖产生了酸，但没有来自二糖醇或山梨糖醇。另外，根据细菌学特征，亚硝酸盐未还原为亚硝酸盐，细菌菌株被鉴定为野油菜黄单胞菌。 vesicatoria（前道奇）。发现病原体受温度影响，最佳温度为30-32°C，但在20和45°C下几乎没有或没有增长。

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