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首页> 外文期刊>Journal of Molecular Genetics >Comparison of Rapid DNA Extraction Techniques for Conventional PCR-RFLP Analysis from Mammalian Whole Blood Cells
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Comparison of Rapid DNA Extraction Techniques for Conventional PCR-RFLP Analysis from Mammalian Whole Blood Cells

机译：用于哺乳动物全血细胞常规PCR-RFLP分析的快速DNA提取技术的比较

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Clean, high molecular weight DNA is pre-requisite for DNA markers. The amount and quality of DNA is a crucial point for all further analysis. A unique advantage of these PCR techniques is the rapid DNA analysis of many animal samples using small quantities of DNA. Thus, a simple and rapid DNA extraction method is needed for studies such as genetic analysis that require large populations. Several methods for minimizing the DNA extraction steps have been reported but they require a large amount of animal tissues. In addition, bleeding and management of sampling and storage of the blood sample in freezers is often difficult due to space constraints. To overcome these problems, some techniques developed a DNA extraction method using the milk or hair root or semen. Researchers compared 4 methods of rapid DNA extraction with isolations of mammalian whole blood samples. DNA extraction methods included boiling, salting out, phenol-chloroform and silica gel procedures. Spectrophotometry and gel monitoring evaluated the DNA yield and purity for the 4 methods. The silica gel and phenol-chloroform methods yielded significantly purity and higher concentration of extracted DNA compared with other DNA extraction methods.
机译：清洁的高分子量DNA是DNA标记的前提。 DNA的数量和质量是所有进一步分析的关键点。这些PCR技术的独特优势是使用少量DNA对许多动物样品进行快速DNA分析。因此，需要简单且快速的DNA提取方法来进行诸如需要大量人口的遗传分析之类的研究。已经报道了使DNA提取步骤最小化的几种方法，但是它们需要大量的动物组织。另外，由于空间的限制，通常难以对血液进行冷冻以及在冷冻机中进行采样以及在血液中进行采样和存储的管理。为了克服这些问题，一些技术开发了一种使用牛奶或发根或精液的DNA提取方法。研究人员比较了4种快速DNA提取方法与分离哺乳动物全血样品的方法。 DNA提取方法包括煮沸，盐析，苯酚氯仿和硅胶程序。分光光度法和凝胶监测评估了这4种方法的DNA产量和纯度。与其他DNA提取方法相比，硅胶和苯酚-氯仿方法可产生明显的纯度和更高的提取DNA浓度。

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