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首页> 外文期刊>Journal of Natural Sciences Research >Phenotypic detection of resistance in Staphylococcus aureus isolates: Detection of (mec A and fem A) gene in methicillin resistant Staphylococcus aureus (MRSA) by Polymerase Chain Reaction
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Phenotypic detection of resistance in Staphylococcus aureus isolates: Detection of (mec A and fem A) gene in methicillin resistant Staphylococcus aureus (MRSA) by Polymerase Chain Reaction

机译：表型检测金黄色葡萄球菌的耐药性：通过聚合酶链反应检测耐甲氧西林的金黄色葡萄球菌（MRSA）中的（mec A和fem A）基因

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摘要

Background: The drug resistant phenomenon is being worldwide concern especially in the last 20 years. Among the most threatening antibiotic-resistant pathogens known are strains of methicillin-resistant S. aureus ? (MRSA), they are resistant to ?-lactams and other cell-wall-active agents. In many developing countries, the situation appears gloomy due to inadequate or poor implementation of policy on infection control, lack of political will, inadequate resources including shortage of skilled manpower, poor motivation of health care workers and researchers. Objective: The present study tries to describe an accurate and quick detection for of clinically relevant antibiotic resistance gene of Staphylococcus aureus using PCR technique. Methodology: The mec A gene was amplified to characterize MRSA isolates at species level. The S. aureus ? isolates were analyzed for their susceptibility to different classes of antibiotics using the disk diffusion method. Results: Of the total 429 Staphylococcus aureus isolated, 114 (26.54%) strains were MRSA. MSRA strains were selected for PCR assay. Eighty one MRSA strains (71.05%) were mecA gene positive and thirty three (28.95%) MRSA strains were mecA negative visualized on 1% agarose gel electrophoresis. Conclusions: The PCR assay was rapid and accurate procedure for the detection mec A gene of MRSA strains as compared to the conventional methods like cutler, biochemical and microscopically since the time was taken is less and can help efficiently in infection management. Keywords: mec A, fem A, MRSA, SCC, PBP2a, PCR, genes, Staphylococcus aureus
机译：背景：耐药现象已引起全球关注，尤其是在最近20年中。在已知的最具威胁性的抗生素耐药性病原体中，有耐甲氧西林的金黄色葡萄球菌菌株。 （MRSA），它们对β-内酰胺和其他细胞壁活性剂有抵抗力。在许多发展中国家，由于关于感染控制的政策执行不力或执行不力，缺乏政治意愿，资源不足（包括熟练人力不足），医护人员和研究人员的积极性较差，情况似乎令人沮丧。目的：本研究试图描述一种利用PCR技术准确，快速地检测临床相关的金黄色葡萄球菌抗生素耐药基因的方法。方法：扩增mec A基因，以在物种水平上表征MRSA分离株。金黄色葡萄球菌？使用圆盘扩散法分析了分离株对不同种类抗生素的敏感性。结果：在总共分离出的429株金黄色葡萄球菌中，有114株（26.54％）是MRSA。选择MSRA菌株用于PCR测定。在1％琼脂糖凝胶电泳中可见81例MRSA菌株（71.05％）为mecA基因阳性，而33例（28.95％）MRSA菌株为mecA阴性。结论：与传统的刀剪法，生化法和显微法相比，PCR法检测MRSA菌株mec A基因的方法快速，准确，因为所花费的时间更少，可以有效地帮助进行感染管理。关键词：mec A，fem A，MRSA，SCC，PBP2a，PCR，基因，金黄色葡萄球菌

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