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首页> 外文期刊>Journal of New Developments in Chemistry >Development of Poly-ε-Caprolactone Based Nanoadjuvant for Effective Vaccination Against Tuberculosis
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Development of Poly-ε-Caprolactone Based Nanoadjuvant for Effective Vaccination Against Tuberculosis

机译：以聚ε-己内酯为基础的纳米佐剂的开发，可有效预防肺结核

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The aim of the study was to synthesize sub-100nm poly-ε-caprolactone nanoparticles (PCL NP), load them with the mycobacterial protein, ESAT 6 and study the resulting immune responses in CD4+ and CD8+ T cells when incubated with human peripheral blood monocyte derived macrophages that had internalized the PCL NP. The synthesized PCL NP were characterized for size, shape and charge. They were found to be about 60nm in size with spherical shape. MTT assay revealed that the particles were perfectly biocompatible when tested in vitro on THP1 human monocytic cell line. The particles had a slow protein release kinetics and did not degrade appreciably even after 30 days in buffer solution. ELISA was used to quantify the cytokine response of CD4+ and CD8+ T cells when incubated with the monocyte derived macrophages as antigen presenting cells. The result of antigen presentation assay revealed that the antigen loaded PCL NP enhanced Th1 and CD8+ T cell responses significantly compared to the pure antigen. Thus we conclude that PCL NP of 60nm size can be effectively tested as a vaccine adjuvant with resulting activation of Th1/Th2 immunity as well as cytotoxic T cell response.">展开▼
机译：该研究的目的是合成低于100nm的聚ε-己内酯纳米颗粒（PCL NP），将分枝杆菌蛋白ESAT 6加载到纳米颗粒中，并研究与人外周血单核细胞孵育后在CD4 +和CD8 + T细胞中产生的免疫反应衍生的巨噬细胞已经内化了PCL NP。表征了合成的PCL NP的大小，形状和电荷。发现它们的大小约为60nm，球形。 MTT分析显示，当在THP1人单核细胞系上进行体外测试时，这些颗粒具有完美的生物相容性。颗粒具有缓慢的蛋白质释放动力学，即使在缓冲溶液中放置30天也不会明显降解。当与单核细胞衍生的巨噬细胞作为抗原呈递细胞孵育时，ELISA用于定量CD4 +和CD8 + T细胞的细胞因子反应。抗原呈递测定的结果表明，与纯抗原相比，载有抗原的PCL NP显着增强了Th1和CD8 + T细胞反应。因此，我们得出结论，可以有效测试60nm大小的PCL NP作为疫苗佐剂，从而激活Th1 / Th2免疫力以及产生细胞毒性T细胞反应。“>

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