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首页> 外文期刊>Journal of New Developments in Chemistry >Phytohemagglutinin and Light-Induced Charge Density Effects on Plasma Membrane of PlectonemaBoryanum
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Phytohemagglutinin and Light-Induced Charge Density Effects on Plasma Membrane of PlectonemaBoryanum

机译：植物血凝素和光诱导的电荷密度对柏单胞菌 花草的质膜的影响

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摘要

Effects of the plant lectin phytohemagglutinin M (PHA-M) and illumination on the electrophoretic mobility (EPM) and 90°-light scattering of suspension of spheroplasts obtained by lysis of the unicellular diazothrophic cyanobacteriumPlectonemaboryanumwere studied. Cells cultures were grown on media with different iron content: an iron-deficient (denominated as “Fe-starved” culture), an iron-sufficient (“Fe-sufficient”), and a twentyfold iron excess medium (“Fe-excess”). Lectin addition led to a decrease of the membrane charge density and aggregation of the vesicles. Our results indicate significant influence of the Fe2+ and illumination on the lectin-induced decreasing of EPM and light scattering intensity.
机译：研究了植物凝集素植物血凝素M（PHA-M）和光照对单细胞重氮嗜蓝细菌Plectonemaboryanum裂解获得的原生质球悬浮液的电泳迁移率（EPM）和90°光散射的影响。细胞培养物在具有不同铁含量的培养基上生长：缺铁（称为“ Fe饥饿”培养物），缺铁（“ Fe充足”）和二十倍铁过量培养基（“ Fe过量”） ）。添加凝集素导致膜电荷密度降低和囊泡聚集。我们的结果表明，Fe2 +和光照对凝集素诱导的EPM和光散射强度下降的影响很大。

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