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首页> 外文期刊>Journal of neuroinflammation >A comparative review of cell culture systems for the study of microglial biology in Alzheimer’s disease
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A comparative review of cell culture systems for the study of microglial biology in Alzheimer’s disease

机译：用于阿尔茨海默氏病小胶质生物学研究的细胞培养系统的比较综述

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Over the past two decades, it has become increasingly apparent that Alzheimer’s disease neuropathology is characterized by activated microglia (brain resident macrophages) as well as the classic features of amyloid plaques and neurofibrillary tangles. The intricacy of microglial biology has also become apparent, leading to a heightened research interest in this particular cell type. Over the years a number of different microglial cell culturing techniques have been developed to study either primary mammalian microglia, or immortalized cell lines. Each microglial system has advantages and disadvantages and should be selected for its appropriateness in a particular research context. This review summarizes several of the most common microglial cell culture systems currently being employed in Alzheimer’s research including primary microglia; BV2 and N9 retroviral immortalized microglia; human immortalized microglia (HMO6); and spontaneously immortalized rodent microglial lines (EOC lines and HAPI cells). Particularities of cell culture requirements and characteristics of microglial behavior, especially in response to applied inflammogen stimuli, are compared and discussed across these cell types.
机译：在过去的二十年中，越来越明显的是，阿尔茨海默氏病的神经病理学特征是活化的小胶质细胞（常驻于大脑的巨噬细胞）以及淀粉样斑块和神经原纤维缠结的经典特征。小胶质细胞生物学的复杂性也已变得显而易见，从而导致对该特定细胞类型的研究兴趣增强。多年来，已经开发出许多不同的小胶质细胞培养技术来研究原代哺乳动物小胶质细胞或永生化细胞系。每个小胶质细胞系统都有其优点和缺点，因此应在特定的研究背景下对其适当性进行选择。这篇综述总结了阿尔茨海默氏症研究中目前使用的几种最常见的小胶质细胞培养系统，包括原发性小胶质细胞; BV2和N9逆转录病毒永生的小胶质细胞;人类永生小胶质细胞（HMO6）;自发永生的啮齿动物小神经胶质细胞系（EOC系和HAPI细胞）。比较并讨论了这些细胞类型对细胞培养要求的特殊性和小胶质细胞行为的特征，特别是对所施加的炎症原刺激的响应。

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