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首页> 外文期刊>Journal of Natural Sciences Research >The Transformational Role of Polymerase Chain Reaction (PCR) in Environmental Health Research
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The Transformational Role of Polymerase Chain Reaction (PCR) in Environmental Health Research

机译:聚合酶链反应(PCR)在环境健康研究中的转化作用

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The Polymerase chain reaction (PCR) has transformed the entire world of scientific research, especially the biological sciences and its related disciplines. It is a technique that allows the exponential amplification of target DNA sequences. Polymerase chain reaction has tremendously wide application in diverse fields of study. Some of these include, Classification of organisms, Archaeological studies, Mutation detection, DNA Sequencing and phylogenetic studies, Diagnosis of medical disorders and diseases, Cancer research, HIV detection, Detection of pathogens (both plant and animals), Genetic fingerprinting, Drug discovery and toxicity testing, Genetic engineering, Pre-natal diagnosis of genetic disorders, Environmental pollution monitoring and control, Environmental and public health monitoring, forensic and criminology, etc. Whilst, the use of Polymerase chain reaction procedures are widely adopted by industries, laboratories and research centres in developed countries, developing countries such as Nigeria are yet to harness the tremendous benefit of this revolutionary scientific tool of molecular biology. Hence this paper attempts to elucidate the usefulness of PCR-based research as it relates to Environmental health in particular. Unlike the traditional use of culture media in isolation and detection of pathogenic organisms in environmental samples, which are often fastidious, slow and sometimes grossly misleading, the use of PCR-based molecular approach is rapid and sensitive and could be used as an alternative method for monitoring environmental health quality. Keywords: PCR, Molecular Biology, Environmental health, DNA
机译:聚合酶链反应(PCR)改变了整个科学研究领域,尤其是生物科学及其相关学科。它是一种允许靶DNA序列指数扩增的技术。聚合酶链式反应在不同的研究领域中有着极为广泛的应用。其中包括:生物体分类,考古学研究,突变检测,DNA测序和系统发育研究,医学疾病和疾病的诊断,癌症研究,HIV检测,病原体(动植物)检测,基因指纹,药物发现和毒性测试,基因工程,遗传疾病的产前诊断,环境污染监测与控制,环境与公共卫生监测,法医和犯罪学等。同时,聚合酶链反应程序的使用已为工业,实验室和研究机构广泛采用发达国家的研究中心,尼日利亚等发展中国家尚未利用这种革命性的分子生物学科学工具带来的巨大收益。因此,本文试图阐明基于PCR的研究的有用性,因为它特别涉及环境健康。与传统的使用培养基来分离和检测环境样品中的病原生物通常是挑剔的,缓慢的,有时是严重的误导性的方法不同,基于PCR的分子方法的使用是快速而灵敏的,可以用作替代方法监测环境健康质量。关键字:PCR,分子生物学,环境健康,DNA

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