• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Oral Microbiology >A dysbiotic mycobiome dominated by Candida albicans is identified within oral squamous-cell carcinomas
【24h】

A dysbiotic mycobiome dominated by Candida albicans is identified within oral squamous-cell carcinomas

机译：在口腔鳞状细胞癌中鉴定出一种由白色念珠菌控制的代谢不良菌群。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study was to characterize the mycobiome associated with oral squamous-cell carcinoma (OSCC). DNA was extracted from 52 tissue biopsies (cases: 25 OSCC; controls: 27 intra-oral fibro-epithelial polyps [FEP]) and sequenced for the fungal internal transcribed spacer 2 region using Illumina? 2?x300bp chemistry. Merged reads were classified to species level using a BLASTN-algorithm with UNITE’s named species sequences as reference. Downstream analyses were performed using QIIME??and linear discriminant analysis effect size. A total of 364 species representing 160 genera and two phyla (Ascomycota and Basidiomycota) were identified, with?Candida?and?Malassezia?making up 48% and 11% of the average mycobiome, respectively. However, only five species and four genera were detected in ≥50% of the samples. The species richness and diversity were significantly lower in OSCC. Genera?Candida, Hannaella, and?Gibberella?were overrepresented in OSCC; Alternaria?and?Trametes?were more abundant in FEP....
机译：这项研究的目的是表征与口腔鳞状细胞癌（OSCC）相关的菌群。从52个组织活检组织（病例：25个OSCC;对​​照：27个口腔内纤维上皮息肉[FEP]）中提取DNA，并使用Illumina®对真菌内部转录间隔区2区域进行测序。 2？x300bp化学反应。合并后的读段使用BLASTN算法（以UNITE命名的物种序列作为参考）分类到物种级别。下游分析使用QIIMEβ和线性判别分析效应大小进行。总共鉴定出代表160个属和两个门的364种（子囊菌和担子菌属），其中“ Candida”和“ Malassezia”分别占平均菌群的48％和11％。但是，在≥50％的样本中仅检测到五个物种和四个属。 OSCC的物种丰富度和多样性明显较低。属Candida，Hannaella和Gibberella在OSCC中人数过多。 FEP中的链格孢属和Trametes更丰富。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号