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Oral microbial profiles of individuals with different levels of sugar intake

机译:不同糖摄入水平个体的口腔微生物特征

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The aim was to compare the oral microbial profiles in young adults with an intake of free sugars above or below the current recommendations by the WHO for sugar consumption. Seventy subjects completed a Quantitative Food Frequency Questionnaire to establish the proportion of free sugars in relation to the total energy intake (% E). Subjects with 5% E (n?=?30) formed the low-sugar group, while those with ≥5% E (n?=?40) were regarded as reference group. Saliva and plaque samples were analyzed by qPCR, and 52 of the plaque samples were assayed by HOMINGS. The HOMINGS analysis revealed a comparable core microbiota in plaque samples with Streptococcus, Leptotrichia, Actinobaculum, and Veillonella as predominant. No major differences between groups were revealed by α-diversity testing (p?=?0.83), principal component analysis, or correspondence analysis. Higher relative abundance of Streptococcus sobrinus and Prevotella melaninogenica was observed in plaque samples in the reference group. By qPCR, Scard...
机译:目的是比较摄入的游离糖高于或低于世界卫生组织当前糖消费建议的年轻人的口腔微生物状况。七十名受试者完成了《定量食物频率调查表》,以建立游离糖相对于总能量摄入(%E)的比例。 E <5%(n≥30)的受试者组成低糖组,而E≥5%(n≥40)的受试者被视为参考组。通过qPCR分析唾液和噬斑样品,并通过HOMINGS分析52个噬斑样品。 HOMINGS分析显示,菌斑样本中具有相当的核心微生物群,其中以链球菌,细小球菌,放线菌和韦永氏菌为主。 α-多样性检验(p≤0.83),主成分分析或对应分析未显示组间的主要差异。在参考组的菌斑样品中,观察到链球菌和黑色素原球菌的相对丰度较高。通过qPCR,Scard ...

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