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首页> 外文期刊>Journal of Oral Microbiology >Candida albicans alters the bacterial microbiome of early in vitro oral biofilms
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Candida albicans alters the bacterial microbiome of early in vitro oral biofilms

机译：白色念珠菌改变了早期体外口腔生物膜的细菌微生物组

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The yeast Candida albicans is an oral commensal microorganism, occurring in the oral cavity of 50–70% of healthy individuals. Its effect on oral ecology has mostly been studied using dual-species models, which disregards the complex nature of oral biofilms. The aim of this study was to culture C. albicans in a complex model to study its effect on oral biofilms. Biofilms, inoculated using pooled stimulated saliva with or without addition of C. albicans, were grown under anaerobic, aerobic, or aerobic +5% CO2 conditions. Red autofluorescence was quantified using a spectrophotometer and visualized in fluorescence photographs. The microbiome of 5?h biofilms was determined using 16S rDNA sequencing. C. albicans was only able to proliferate in biofilms grown under aerobic conditions. After 48?h, C. albicans did not induce differences in total biofilm formation, lactic acid accumulation (cariogenic phenotype) or protease activity (periodontitis phenotype). In vitro, anaerobically grown biofilms developed...
机译：酵母白色念珠菌是一种口腔共生微生物，存在于50-70％的健康个体的口腔中。它对口腔生态学的影响主要是使用双物种模型研究的，该模型忽略了口腔生物膜的复杂性。这项研究的目的是在复杂的模型中培养白色念珠菌，以研究其对口腔生物膜的影响。在有或没有添加白色念珠菌的情况下，使用合并的刺激唾液接种的生物膜在无氧，有氧或有氧+ 5％CO2条件下生长。使用分光光度计定量红色自发荧光，并在荧光照片中可视化。使用16S rDNA测序确定5？h生物膜的微生物组。白色念珠菌只能在有氧条件下生长的生物膜中增殖。 48小时后，白色念珠菌没有诱导总生物膜形成，乳酸积累（生龋表型）或蛋白酶活性（牙周炎表型）的差异。在体外，厌氧生长的生物膜得以发展。

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