• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Oral Microbiology >Role of outer membrane proteins in serum resistance of A. actinomycetemcomitans and A. aphrophilus
【24h】

Role of outer membrane proteins in serum resistance of A. actinomycetemcomitans and A. aphrophilus

机译：外膜蛋白在A.放线菌和A. Aphrophilus血清抗药性中的作用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this project was to study how outer membrane proteins contribute to serum resistance in A. actinomycetemcomitans and A. aphrophilus.Genes encoding Omp100 (A. actinomycetemcomitans) and OmpA (A. actinomycetemcomitans and A. aphrophilus) were knocked out through homologous recombination.Serum resistance was tested by incubating the bacterial strains in normal human serum (NHS; 50%) for 2h at 37°C. As control heat-inactivated (56°C) NHS was used. LC-MS/MS analysis was used to identify proteins in selected bands from SDS-PAGE.Whereas Omp100 appeared not to contribute significantly to serum resistance, we observed that the ompA mutants of both species had a markedly reduced capability to survive in 50% NHS. In presence of Mg-EGTA the ompA mutants survived clearly better in NHS suggesting that OmpA may act through the classical pathway of the complement system. Interestingly, when incubating A. actinomycetemcomitans ompA mutants in NHS, derivatives were obtained that had regained serum resist...
机译：该项目的目的是研究外膜蛋白如何促进A.放线菌和A.Aphrophilus的血清抗性。通过同源重组敲除编码Omp100（A。放线菌和A.Aphronus）和OmpA（A。放线菌和A.Aphrophilus）的基因通过将细菌菌株在正常人血清（NHS; 50％）中于37°C孵育2小时来测试血清抗药性。作为对照，使用热灭活的（56℃）NHS。使用LC-MS / MS分析从SDS-PAGE中鉴定出选定条带中的蛋白质。虽然Omp100似乎对血清抗性没有显着贡献，但我们观察到这两个物种的ompA突变体在50％NHS中的存活能力均明显降低。在存在Mg-EGTA的情况下，ompA突变体在NHS中的存活明显更好，这表明OmpA可能通过补体系统的经典途径起作用。有趣的是，在NHS中孵育放线放线杆菌的ompA突变体时，获得的衍生物已恢复了血清抗性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号