• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Oral Microbiology >Influence of DNA extraction on oral microbial profiles obtained via 16S rRNA gene sequencing
【24h】

Influence of DNA extraction on oral microbial profiles obtained via 16S rRNA gene sequencing

机译：DNA提取对通过16S rRNA基因测序获得的口腔微生物谱的影响

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background and objectiveThe advent of next-generation sequencing has significantly facilitated characterization of the oral microbiome. Despite great efforts in streamlining the processes of sequencing and data curation, upstream steps required for amplicon library generation could still influence 16S rRNA gene-based microbial profiles. Among upstream processes, DNA extraction is a critical step that could represent a great source of bias. Accounting for bias introduced by extraction procedures is important when comparing studies that use different methods. Identifying the method that best portrays communities is also desirable. Accordingly, the aim of this study was to evaluate bias introduced by different DNA extraction procedures on oral microbiome profiles.DesignFour DNA extraction methods were tested on mock communities consisting of seven representative oral bacteria. Additionally, supragingival plaque samples were collected from seven individuals and divided equally to test two commonly used DNA ex...
机译：背景和目的新一代测序的出现极大地促进了口腔微生物组的表征。尽管在简化测序和数据管理流程方面做出了巨大努力，但扩增子文库生成所需的上游步骤仍可能影响基于16S rRNA基因的微生物谱。在上游过程中，DNA提取是关键步骤，可能代表了很大的偏见。在比较使用不同方法的研究时，考虑提取程序引入的偏差很重要。确定最能描绘社区的方法也是可取的。因此，本研究的目的是评估不同DNA提取方法对口腔微生物组谱引入的偏倚。在包括7种代表性口腔细菌的模拟群落上测试了DesignFour DNA提取方法。此外，从七个人身上收集龈上菌斑样品，并均分以测试两种常用的DNA提取物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号