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首页> 外文期刊>Journal of Oral Microbiology >Pulp and plaque microbiotas of children with severe early childhood caries
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Pulp and plaque microbiotas of children with severe early childhood caries

机译：患有严重幼儿龋齿的儿童的纸浆和菌斑微生物区系

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AbstractBackground and objective: Bacterial invasion into pulps of primary teeth can lead to infection and premature tooth loss in children. This pilot study aimed to explore whether the microbiota of carious exposures of dental pulps resembles that of carious dentin or that of infected root canals.Design: Children with severe early childhood caries were studied. Children were consented and extent of caries, plaque, and gingivitis measured. Bacteria were sampled from carious lesion biofilms and vital carious exposures of pulps, and processed by anaerobic culture. Isolates were characterized from partial sequences of the 16S rRNA gene and identified by comparison with taxa in the Human Oral Microbiome Database (http://www.HOMD.org). The microbiotas of carious lesions and dental pulps were compared using univariate and multivariate approaches.Results: The microbiota of cariously exposed pulps was similar in composition to that of carious lesion biofilms except that fewer species/taxa were identified from pu...
机译：摘要背景与目的：细菌侵入乳牙的牙髓可导致儿童感染和牙齿早脱落。这项初步研究旨在探讨龋牙暴露于牙本质的微生物区系与龋齿或被感染的根管的微生物区系。设计：研究了患有严重幼儿龋齿的儿童。征得儿童的同意，并测量龋齿，斑块和牙龈炎的程度。从龋病病变生物膜和牙髓重要龋病暴露中取样细菌，并通过厌氧培养进行处理。从16S rRNA基因的部分序列中鉴定出分离物，并通过与人类口腔微生物组数据库（http://www.HOMD.org）中的分类单元进行比较进行鉴定。结果：龋暴露的牙髓的微生物群与龋病变生物膜的微生物群相似，不同之处在于龋齿和牙髓的微生物群组成与龋病生物膜相似。

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