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首页> 外文期刊>Journal of Pharmaceutical Analysis >Simultaneous determination of nortriptyline hydrochloride and fluphenazine hydrochloride in microgram quantities from low dosage forms by liquid chromatography–UV detection
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Simultaneous determination of nortriptyline hydrochloride and fluphenazine hydrochloride in microgram quantities from low dosage forms by liquid chromatography–UV detection

机译：液相色谱-紫外检测法同时测定低剂型中毫克含量的去甲替林盐酸盐和盐酸氟苯那嗪

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摘要

A novel method for the simultaneous high-performance liquid chromatographic determination of nortriptyline hydrochloride and fluphenazine hydrochloride was developed and validated. Fluvastatin sodium was used as internal standard. The determination was performed on a Hypersil Gold C 8 column (250 mm × 4.6 mm i.d., 5μm particle size) at 25°C; the mobile phase, consisting of a mixture of formic acid (0.1M, pH 2.16)-methanol (33:67, v / v), was delivered at a flow rate of 1.1mL/min and detector wavelength at 251nm. The retention time of nortriptyline, fluphenazine and fluvastatin was found to be 5.11, 8.05 and 11.38min, respectively. Linearity ranges were 5.0–1350.0 and 10.0–1350.0μg/mL with limit of detection values of 0.72 and 0.31μg/mL, for nortriptyline and fluphenazine, respectively. Results of assay and recovery studies were statistically evaluated for its accuracy and precision. Correlation coefficients ( r 2 ) of the regression equations were greater than 0.999 in all cases. According to the validation results, the proposed method was found to be specific, accurate, precise and could be applied to the simultaneous quantitative analysis of nortriptyline and fluphenazine.
机译：建立并验证了同时测定盐酸去甲替林和盐酸氟苯那嗪的高效液相色谱新方法。氟伐他汀钠用作内标。测定是在25°C的Hypersil Gold C 8色谱柱（250 mm×4.6 mm i.d.，5μm粒径）上进行的;流动相由甲酸（0.1M，pH 2.16）-甲醇（33:67，v / v）的混合物组成，流速为1.1mL / min，检测器波长为251nm。去甲替林，氟奋乃静和氟伐他汀的保留时间分别为5.11、8.05和11.38min。去甲替林和氟奋乃静的线性范围分别为5.0–1350.0和10.0–1350.0μg / mL，检出限分别为0.72和0.31μg/ mL。对测定和回收率研究的结果进行统计学评估，以评估其准确性和准确性。在所有情况下，回归方程的相关系数（r 2）均大于0.999。根据验证结果，该方法具有特异性，准确度，精确性，可用于去甲替林和氟奋乃静的同时定量分析。

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