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首页> 外文期刊>Journal of orthopaedics and traumatology: official journal of the Italian Society of Orthopaedics and Traumatology >Association of ex-vivo expanded human mesenchymal stem cells and rhBMP-7 is highly effective in treating critical femoral defect in rats
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Association of ex-vivo expanded human mesenchymal stem cells and rhBMP-7 is highly effective in treating critical femoral defect in rats

机译：体外扩增的人类间充质干细胞与rhBMP-7的结合对治疗大鼠严重股骨缺损非常有效

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Mesenchymal stem cells (MSC), easily culture-expanded from bone marrow, can significantly enhance bone defect healing. Several proteins, such as the bone morphogenetic proteins (BMPs) and in particular BMP-7, are involved in bone formation in vitro and in vivo. In this preclinical study, we evaluated if the association of human MSC (hMSC) with BMP-7 had synergic action on bone healing. Rat femoral defects (n=12) were treated with: autoclaved bone and mononucleated cells (MNC) as control group G1; bone and hMSC, group G2; bone with BMP-7, group G3; bone and hMSC plus BMP-7, group G4. Defect regeneration was evaluated with plain radiographs after 2, 4, 8 and 12 weeks and with histological analysis. We observed organized trabeculae bridging between the osteotomic ends of the host bone in rats treated with the association of hMSC and rhBMP-7. These trabeculae, formed by a core of devitalized tissue surrounded by osteoblasts, osteocytes and osteoclasts, were continuous with a cortical-like structure of bony tissue. Such new bone formation of the group treated with the association of hMSC and rhBMP-7 (G4) was clearly superior compared to rats treated with rhBMP-7 (G2) or hMSC (G3) alone, as shown by radiographic analysis and histological study. The present study suggests that the association of hMSC and BMP-7 is more effective than hMSC or BMP-7 alone in the healing of femoral defects in rats. Further studies with larger samples are required to confirm these results and to evaluate the best dosage.
机译：间充质干细胞（MSC）易于从骨髓培养扩增，可显着增强骨缺损的愈合。几种蛋白质，例如骨形态发生蛋白（BMP），尤其是BMP-7，在体外和体内都参与了骨的形成。在这项临床前研究中，我们评估了人类MSC（hMSC）与BMP-7的结合是否对骨愈合具有协同作用。用高压灭菌的骨和单核细胞（MNC）作为对照组G1治疗大鼠股骨缺损（n = 12）;骨和hMSC，组G2;骨与BMP-7，组G3;骨和hMSC加BMP-7，G4组。在第2、4、8和12周后用平片检查缺陷的再生，并用组织学分析。我们观察到在用hMSC和rhBMP-7联合治疗的大鼠中，有组织的小梁桥接在宿主骨的成骨末端之间。这些骨小梁是由被成骨细胞，骨细胞和破骨细胞包围的失活组织的核心所形成，并与骨组织的皮质样结构连续。通过放射线照相分析和组织学研究显示，与单独用rhBMP-7（G2）或hMSC（G3）治疗的大鼠相比，接受hMSC和rhBMP-7（G4）治疗的组的这种新骨形成明显更好。本研究表明，hMSC和BMP-7的联合在单独治疗大鼠股骨缺损方面比单独的hMSC或BMP-7更有效。需要对更大的样品进行进一步的研究，以确认这些结果并评估最佳剂量。

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