Background class='Para'>Use of antibiotic-loaded acrylic bone cement to treat orthopaedic infections continues to remain popular, but resistance to routinely used antibiotics has'/> In vitro study of elution kinetics and bio-activity of meropenem-loaded acrylic bone cement
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首页> 外文期刊>Journal of orthopaedics and traumatology: official journal of the Italian Society of Orthopaedics and Traumatology >In vitro study of elution kinetics and bio-activity of meropenem-loaded acrylic bone cement
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In vitro study of elution kinetics and bio-activity of meropenem-loaded acrylic bone cement

机译：美罗培南负载的丙烯酸骨水泥洗脱动力学和生物活性的体外研究

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class="Heading">Background class="Para">Use of antibiotic-loaded acrylic bone cement to treat orthopaedic infections continues to remain popular, but resistance to routinely used antibiotics has led to the search for alternative, more effective antibiotics. We studied, in?vitro, the elution kinetics and bio-activity of different concentrations of meropenem-loaded acrylic bone cement. class="Heading">Methods class="Para">Meropenem-loaded bone cement cylinders of different concentrations were serially immersed in normal saline. Elution kinetics was studied by measuring the drug concentration in the eluate, collected at pre-determined intervals, by high-performance liquid chromatography. Bio-activity of the eluate of two different antibiotic concentrations was tested for a period of 3?weeks against each of the following organisms: Staphylococcus aureus ATCC 2593 (MSSA), Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, S.?aureus ATCC 43300 (MRSA) and Klebsiella pneumoniae ATCC 700603 (ESBL). class="Heading">Results class="Para">Meropenem elutes from acrylic bone cement for a period of 3–27?days depending on the concentration of antibiotic. Higher doses of antibiotic concentration resulted in greater elution of the antibiotic. The eluate was found to be biologically active against S.?aureus ATCC 2593 (MSSA), P.?aeruginosa ATCC 27853, E.?coli ATCC 25922 and K.?pneumoniae ATCC 700603 (ESBL) for a period of 3?weeks. class="Heading">Conclusions class="Para">The elution of meropenem is in keeping with typical antibiotic-loaded acrylic bone cement elution characteristics. The use of high-dose meropenem-loaded acrylic bone cement seems to be an attractive option for treatment of resistant Gram-negative orthopaedic infections but needs to be tested in?vivo.
机译：class =“ Heading”>背景 class =“ Para”>使用载有抗生素的丙烯酸骨水泥治疗骨科感染仍然很流行，但是对常规使用的抗生素的耐药性促使人们寻求替代的，更有效的抗生素。我们体外研究了不同浓度的美罗培南负载的丙烯酸骨水泥的洗脱动力学和生物活性。 class =“ Heading”>方法 class =“ Para”>将负载美罗培南的不同浓度的骨水泥圆柱体依次浸入生理盐水中。通过测定洗脱液中的药物浓度（通过预定的时间间隔，通过高效液相色谱法进行收集）来研究洗脱动力学。测试了两种不同抗生素浓度的洗脱液对下列每种生物的生物活性，持续了3周时间：金黄色葡萄球菌 ATCC 2593（MSSA），粪肠球菌 ATCC 29212，绿脓杆菌 ATCC 27853，大肠杆菌 ATCC 25922，金黄色葡萄球菌 ATCC 43300（MRSA）和肺炎克雷伯菌 ATCC 700603（ESBL）。 class =“ Heading “>结果 class =” Para“>美罗培南从丙烯酸骨水泥中洗脱3–27天，具体取决于抗生素的浓度。更高剂量的抗生素浓度导致更大的抗生素洗脱。发现洗脱液对 S.?aureus ATCC 2593（MSSA）具有生物活性， P.?aruginosa ATCC 27853， 大肠杆菌 ATCC 25922和肺炎克雷伯菌ATCC 700603（ESBL），持续3周。 class =“ Heading”>结论 class =“ Para”>美罗培南的洗脱与典型的载有抗生素的丙烯酸骨水泥的洗脱特征保持一致。使用大剂量美罗培南的丙烯酸骨水泥似乎是治疗耐药革兰氏阴性骨科感染的有吸引力的选择，但需要在体内进行测试。

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