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首页> 外文期刊>Journal of Pharmaceutical Analysis >Cytotoxicity and cellular imaging of quantum dots protected by polyelectrolyte
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Cytotoxicity and cellular imaging of quantum dots protected by polyelectrolyte

机译:聚电解质保护的量子点的细胞毒性和细胞成像

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The nanocomposites of poly-diallyldimethylammonium chloride (PDADMAC) and CdTe quantum dots (QDs) (i.e. QDs-PDADMAC nanocomposites) have been prepared based on electrostatic interaction and their fluorescence stability in aqueous solution has been investigated. MTT method (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide method) was used to study their cytotoxicity and A549 lung cancer cell as a model cell was also used to evaluate their cellular imaging. It was shown that the fluorescence stability of QDs-PDADMAC nanocomposites was much better than that of bare QDs both in aqueous solution and cell. Meanwhile, QDs-PDADMAC nanocomposites display very low cytotoxicity in the low concentrations and better staining ability compared with QDs. QDs-PDADMAC nanocomposites will have great advantage on the cell analysis detection and imaging.
机译:基于静电相互作用制备了聚二烯丙基二甲基氯化铵(PDADMAC)和CdTe量子点(QDs)的纳米复合材料(即QDs-PDADMAC纳米复合材料),并研究了它们在水溶液中的荧光稳定性。 MTT法(3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化法)用于研究其细胞毒性,A549肺癌细胞作为模型细胞也用于评估其细胞成像。结果表明,无论是在水溶液中还是在细胞中,QDs-PDADMAC纳米复合材料的荧光稳定性都比裸QDs更好。同时,与QDs相比,QDs-PDADMAC纳米复合物在低浓度下显示出非常低的细胞毒性,并且具有更好的染色能力。 QDs-PDADMAC纳米复合材料将在细胞分析检测和成像方面具有很大的优势。

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