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首页> 外文期刊>Journal of Pharmacopuncture >Relative Apoptosis-inducing Potential of Homeopa-thic Condurango 6C and 30C in H460 Lung Cancer Cells In vitro
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Relative Apoptosis-inducing Potential of Homeopa-thic Condurango 6C and 30C in H460 Lung Cancer Cells In vitro

机译：体外诱导H460肺癌细胞相对凋亡诱导的顺势性Condurango 6C和30C的潜力。

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Objectives: In homeopathy, it is claimed that more homeopathically-diluted potencies render more protective/curative effects against any disease condition. Potentized forms of Condurango are used successfully to treat digestive problems, as well as esophageal and stomach cancers. However, the comparative efficacies of Condurango 6C and 30C, one diluted below and one above Avogadro’s limit (lacking original drug molecule), respectively, have not been critically analyzed for their cell-killing (apoptosis) efficacy against lung cancer cells in vitro, and signalling cascades have not been studied. Hence, the present study was undertaken. Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide (MTT) assays were conducted on H460-non-small-cell lung cancer (NSCLC) cells by using a succussed ethyl alcohol vehicle (placebo) as a control. Studies on cellular morphology, cell cycle regulation, generation of reactive oxygen species (ROS), changes in mitochondrial membrane potential (MMP), and DNA-damage were made, and expressions of related signaling markers were studied. The observations were done in a “blinded” manner. Results: Both Condurango 6C and 30C induced apoptosis via cell cycle arrest at subG0/G1 and altered expressions of certain apoptotic markers significantly in H460 cells. The drugs induced oxidative stress through ROS elevation and MMP depolarization at 18-24 hours. These events presumably activated a caspase-3-mediated signalling cascade, as evidenced by reverse transcriptase- polymerase chain reaction (RT-PCR), western blot and immunofluorescence studies at a late phase (48 hours) in which cells were pushed towards apoptosis. Conclusion: Condurango 30C had greater apoptotic effect than Condurango 6C as claimed in the homeopathic doctrine.
机译：目标：在顺势疗法中，据称顺势疗法稀释的药效对任何疾病都具有更大的保护/疗效。强化形式的Condurango已成功用于治疗消化系统疾病以及食道癌和胃癌。然而，尚未严格分析Condurango 6C和30C的相对功效，分别稀释至低于和低于Avogadro的限值（缺乏原始药物分子）的一倍，并没有针对其在体外对肺癌细胞的杀伤（凋亡）功效进行严格分析，并且尚未研究信号级联。因此，进行了本研究。方法：采用乙醇提取法，对H460-非小细胞肺癌（NSCLC）细胞进行3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化物（MTT）测定车辆（安慰剂）作为对照。研究了细胞形态，细胞周期调控，活性氧（ROS）的产生，线粒体膜电位（MMP）的变化和DNA损伤，并研究了相关信号标记的表达。观察是以“盲目的”方式进行的。结果：Condurango 6C和30C均通过在subG0 / G1处的细胞周期停滞诱导凋亡，并显着改变了H460细胞中某些凋亡标记的表达。该药物在18-24小时通过ROS升高和MMP去极化诱导氧化应激。这些事件可能激活了caspase-3介导的信号级联反应，这在后期（48小时）中被逆转录酶-聚合酶链反应（RT-PCR），蛋白质印迹和免疫荧光研究所证实，在该阶段细胞被推向凋亡。结论：顺势疗法理论中所述，Condurango 30C的凋亡作用大于Condurango 6C。

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