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首页> 外文期刊>Journal of Pharmacy and Pharmaceutical Sciences >Intracellular Uptake Mechanism of Lutein in Retinal Pigment Epithelial Cells
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Intracellular Uptake Mechanism of Lutein in Retinal Pigment Epithelial Cells

机译：叶黄素在视网膜色素上皮细胞中的细胞内摄取机制

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Purpose. Lutein is a carotenoid mainly found in green leafy vegetables and is located in the macula lutea in the human eye. It has received much attention recently due to its preventive effect on age-related macular degeneration, and it has been consumed as a supplement. However, little information about the pharmacokinetic properties of lutein is available. Detailed knowledge of pharmacokinetic properties of lutein is needed for the development of pharmaceutics. In this study, we focused on the macular accumulation of lutein and investigated the uptake mechanism into human retinal pigment epithelial cells. Methods. ARPE-19 cells were used for the study on the accumulation mechanism of lutein. The concentration of lutein was determined using an HPLC system. Involvement of scavenger class B type 1 (SR-B1) in the accumulation of lutein in ARPE-19 cells was suggested from the results of an inhibition study using block lipid transport 1 (BLT-1), a selective inhibitor of SR-B1. To investigate the involvement of SR-B1 in more detail, small interfering RNA (siRNA) was transfected and the mRNA and protein expression levels of SR-B1 were assessed by quantitative real-time reverse transcription polymerase chain reaction and Western blotting, respectively. Results. We confirmed a sufficient siRNA knockdown effect in both mRNA and protein expression levels of SR-B1. We then found that lutein uptake was significantly decreased by siRNA knockdown of SR-B1. Conclusion. The uptake of lutein was significantly decreased by 40% compared with the control uptake level. This suggested that active transport of lutein into ARPE-19 cells is mainly via SR-B1, given the result that lutein uptake at 4oC was about 40% less that that at 37oC. This article is open to POST-PUBLICATION REVIEW . Registered readers (see “For Readers”) may comment by clicking on on the issue’s contents page.
机译：目的。叶黄素是一种类胡萝卜素，主要存在于绿叶蔬菜中，位于人眼的黄斑区。由于其对与年龄有关的黄斑变性的预防作用，近来受到了广泛的关注，并且已被作为补充品消费。但是，关于叶黄素的药代动力学特性的信息很少。叶黄素的药代动力学特性的详细知识是药物开发的需要。在这项研究中，我们专注于叶黄素的黄斑积聚，并研究了人类视网膜色素上皮细胞的摄取机制。方法。 ARPE-19细胞用于研究叶黄素的积累机制。叶黄素的浓度使用HPLC系统测定。使用阻断性脂质转运蛋白1（BLT-1）（一种选择性的SR-B1抑制剂）进行的抑制研究结果表明，清除剂B类1型（SR-B1）参与了ARPE-19细胞中叶黄素的积累。为了更详细地研究SR-B1的参与，转染了小干扰RNA（siRNA），并通过实时定量逆转录聚合酶链反应和Western印迹分别评估了SR-B1的mRNA和蛋白表达水平。结果。我们证实了SR-B1的mRNA和蛋白表达水平均具有足够的siRNA敲低效应。然后，我们发现通过SR-B1的siRNA敲除可显着降低叶黄素的摄取。结论。与对照吸收水平相比，叶黄素的吸收显着降低了40％。这表明叶黄素主动转运入ARPE-19细胞主要是通过SR-B1进行的，因为结果是4oC时叶黄素的摄取量比37oC时少约40％。本文对POST-PUBLICATION REVIEW开放。已注册的读者（请参阅“针对读者”）可以通过单击问题的内容页面来发表评论。

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