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首页> 外文期刊>Journal of Pharmacy and Pharmaceutical Sciences >Enhanced Prokaryotic Expression of Dengue Virus Envelope Protein
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Enhanced Prokaryotic Expression of Dengue Virus Envelope Protein

机译：登革热病毒包膜蛋白的原核表达增强

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Purpose. To highlight the expression and purification of the recombinant dengue virus type-1 antigen exploiting the codon optimized full length envelope for increased yield in E. coli . Methods. A 6x His tag was inserted at the C terminus to facilitate purification. The purified protein was recognized in Western blot by Monoclonal antibody specific for the tag. The in vitro refolded recombinant protein was used to immunize mice for the development of hybridomas and also analyzed for its biological functionality with heparan sulfate binding assay. Results . The polyclonal anti-sera from the immunized mice were found to recognize the envelope protein thereby establishing the immunogenicity of the protein. Conclusion . The purified envelope protein could potentially be used towards dengue diagnostics and vaccine development efforts. This article is open to POST-PUBLICATION REVIEW . Registered readers (see “For Readers”) may comment by clicking on on the issue’s contents page.
机译：目的。为了突出表达和纯化重组登革病毒1型抗原，利用密码子优化的全长包膜在大肠杆菌中提高产量。方法。在C末端插入6x His标签以促进纯化。纯化的蛋白质在Western blot中被标签特异的单克隆抗体识别。使用体外重折叠的重组蛋白免疫小鼠杂交瘤的发展，并用硫酸乙酰肝素结合试验分析其生物学功能。结果。发现来自免疫小鼠的多克隆抗血清识别包膜蛋白，从而建立了蛋白的免疫原性。结论。纯化的包膜蛋白可潜在地用于登革热诊断和疫苗开发工作。本文对POST-PUBLICATION REVIEW开放。已注册的读者（请参阅“针对读者”）可以通过单击问题的内容页面来发表评论。

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