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首页> 外文期刊>Journal of structural and functional genomics >Large-scale Transient Transfection of Mammalian Cells: A Newly Emerging Attractive Option for Recombinant Protein Production
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Large-scale Transient Transfection of Mammalian Cells: A Newly Emerging Attractive Option for Recombinant Protein Production

机译：哺乳动物细胞的大规模瞬时转染：重组蛋白生产的一种新兴的有吸引力的选择。

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摘要

Mammalian expression systems have an undisputed long-standing and very successful history for the generation of recombinant proteins, mainly as biopharmaceuticals. However, for use as ‘tool proteins’ in, e.g. assay development and screening, for structure elucidation and as antigens these expression systems were generally regarded as being cumbersome, tedious and expensive. This bias has largely been overcome with the very recent development of large-scale transient transfection (LST) approaches. Especially the HEK.EBNA expression system described here has contributed significantly to this success. The simplicity and speed of this approach compares well with expression trials using the widely applied Baculovirus/insect cell system. In addition, proteins generated in mammalian cells are usually correctly folded, fully processed and functionally active.
机译：哺乳动物表达系统具有无可争议的长期成功的历史，可用于重组蛋白的生产，主要是作为生物药物。但是，例如，用作“工具蛋白”对于结构阐明和作为抗原的分析开发和筛选，这些表达系统通常被认为笨重，繁琐且昂贵。随着大规模瞬时转染（LST）方法的最新发展，这种偏见已得到很大程度的克服。尤其是此处描述的HEK.EBNA表达系统为这一成功做出了重要贡献。这种方法的简单性和速度与使用广泛应用的杆状病毒/昆虫细胞系统进行的表达试验相比非常好。另外，在哺乳动物细胞中产生的蛋白质通常被正确折叠，充分加工并具有功能活性。

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