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首页> 外文期刊>Journal of Systems Chemistry >Beyond allostery: Catalytic regulation of a deoxyribozyme through an entropy-driven DNA amplifier
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Beyond allostery: Catalytic regulation of a deoxyribozyme through an entropy-driven DNA amplifier

机译：超越变构：通过熵驱动的DNA放大器对脱氧核酶的催化调节

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The programmability and replicability of RNA and DNA have respectively enabled the design and selection of a number of allosteric ribozymes and deoxyribozymes. These catalysts have been adapted to function as signal transducers in biosensors and biochemical reaction networks both in vitro and in vivo . However, allosteric control of nucleic acid catalysts is currently limited by the fact that one molecule of effector (input) generally regulates at most one molecule of ribozyme or deoxyribozyme (output). In consequence, allosteric control is usually inefficient when the concentration of input molecules is low. In contrast, catalytic regulation of protein enzymes, as in protein phosphorylation cascades, generally allows one input molecule (e.g., one kinase molecule) to regulate multiple output molecules (e.g., kinase substrates). Achieving such catalytic signal amplification would also be of great utility for nucleic acid circuits. Here we show that allosteric regulation of nucleic acid enzymes can be coupled to signal amplification in an entropy-driven DNA circuit. In this circuit, kinetically trapped DNA logic gates are triggered by a specific sequence, and upon execution generate a peroxidase deoxyribozyme that converts a colorless substrate (ABTS) into a green product (ABTS?+). This scheme provides a new paradigm for the design of enzyme-free biosensors for point-of-care diagnostics.
机译：RNA和DNA的可编程性和可复制性分别使得能够设计和选择许多变构核酶和脱氧核酶。这些催化剂已经适应了体内外的生物传感器和生化反应网络中的信号换能器的作用。然而，核酸催化剂的变构控制目前受到以下事实的限制：一分子效应物（输入）通常调节至多一分子核酶或脱氧核酶（输出）。结果，当输入分子的浓度低时，变构控制通常是无效的。相反，如在蛋白质磷酸化级联反应中一样，对蛋白质酶的催化调节通常允许一个输入分子（例如一个激酶分子）来调节多个输出分子（例如激酶底物）。实现这种催化信号放大对于核酸电路也将具有很大的实用性。在这里，我们显示了核酸酶的变构调节可以耦合到熵驱动的DNA电路中的信号放大。在该电路中，由特定序列触发动力学捕获的DNA逻辑门，并在执行时生成过氧化物酶脱氧核酶，该酶​​将无色底物（ABTS）转换为绿色产物（ABTS ？ + ）。该方案为现场护理诊断用无酶生物传感器的设计提供了新的范例。

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