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首页> 外文期刊>Journal of the Brazilian Chemical Society >Sensitive Estrogens Determination in Wastewater Samples by HPLC and Fluorescence Detection
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Sensitive Estrogens Determination in Wastewater Samples by HPLC and Fluorescence Detection

机译：HPLC和荧光检测灵敏测定废水样品中的雌激素

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A sensitive high-performance liquid chromatography-fluorescence detection method (HPLC-FLD) was developed and validated for 17?2-estradiol (E2) and 17?±-ethinylestradiol (EE2) determination in wastewater samples. Sample preparation was performed by solid-phase extraction (SPE) on Hypersep C18 cartridges. Conditioning and elution solvents and sample volume were investigated by a 2 3 factorial design for both analytes. The pre-concentration factor was 2500 times, allowing to quantify 5.0 ng L -1 for both hormones. The SPE-HPLC-FLD method was successfully applied to detect and quantify trace amount of target compounds in raw and treated wastewater samples. E2 was found below limit of quantification in influent and effluent samples. Nevertheless, the recoveries of E2 in standard addition experiments were between 41 and 72%, with relative standard deviations between 4 and 16%. An important matrix effect was observed in the determination of EE2, with fluorescence signal suppression.
机译：开发了一种灵敏的高效液相色谱-荧光检测方法（HPLC-FLD）并验证了该方法可用于废水样品中17β2-雌二醇（E2）和17α±炔雌醇（EE2）的测定。通过在Hypersep C18柱上进行固相萃取（SPE）进行样品制备。通过2 3析因设计研究了两种分析物的条件和洗脱溶剂以及样品量。预浓缩因子是2500倍，可以定量两种激素的5.0 ng L -1。 SPE-HPLC-FLD方法已成功应用于检测和定量原水和处理后废水样品中的痕量目标化合物。在进水和出水样品中发现E2低于定量限。尽管如此，标准添加实验中E2的回收率在41％至72％之间，相对标准偏差在4％至16％之间。在测定EE2时观察到了重要的基质效应，同时抑制了荧光信号。

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