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首页> 外文期刊>Journal of the Brazilian Chemical Society >A Sensitive fluorescent assay for trypsin activity in biological samples using BSA-Au nanoclusters
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A Sensitive fluorescent assay for trypsin activity in biological samples using BSA-Au nanoclusters

机译：使用BSA-Au纳米簇对生物样品中的胰蛋白酶活性进行灵敏的荧光测定

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摘要

A novel, simple, sensitive, and selective fluorometric method was developed for measuring trypsin in biological samples in this article. The method was based upon measuring the quenching of the fluorescence intensity of the bovine serum albumin (BSA) stabilized Au nanoclusters by enzymatic proteolysis. The calibration plot for trypsin was achieved over the concentration range 1-60 nmol L-1 with a correlation coefficient of 0.995 and a limit of detection of 0.6 nmol L-1. The method was also used satisfactorily for the assessment of the trypsin activity and the results showed that the Michaelis-Menten (Km) and catalytic (Kcat) constant values of trypsin for BSA-Au nanoclusters substrate were 1.6×10-5 mol L-1 and 3.8 s-1 at 37 oC, respectively. This enzyme biosensor is of considerable interest due its promise for simple procedure and the established method has great potential in detection of other proteases in clinical diagnostics of various diseases.
机译：本文开发了一种新颖，简单，灵敏且选择性的荧光法，用于测量生物样品中的胰蛋白酶。该方法基于通过酶促蛋白水解测量牛血清白蛋白（BSA）稳定的Au纳米簇的荧光强度的猝灭。在1-60 nmol L-1的浓度范围内获得了胰蛋白酶的校准图，相关系数为0.995，检出限为0.6 nmol L-1。该方法还可以令人满意地用于评估胰蛋白酶的活性，结果表明，胰蛋白酶的BBS-Au纳米簇底物的Michaelis-Menten（Km）和催化（Kcat）常数为1.6×10-5 mol L-1和在3.7 oC时为3.8 s-1。该酶生物传感器由于其简单的操作前景而备受关注，并且所建立的方法在检测各种疾病的临床诊断中的其他蛋白酶方面具有巨大的潜力。

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