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首页> 外文期刊>Journal of the Brazilian Chemical Society >Differentiation of Toxic and Non-Toxic Leaves of Jatropha curcas L. Genotypes by Leaf Spray Mass Spectrometry
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Differentiation of Toxic and Non-Toxic Leaves of Jatropha curcas L. Genotypes by Leaf Spray Mass Spectrometry

机译：叶片喷雾质谱法鉴别麻疯树基因型有毒和无毒叶片

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Jatropha curcas L. is an oil crop that has been studied as a potential source of biodiesel. A high protein pie is produced as a byproduct of the biodiesel production, which could be used as animal feed. However, the pie has toxic compounds, as phorbol esters and other toxins, which prevents the use as animal feed. For this reason, Embrapa (Brazilian Agricultural Research Corporation) has been working in genetic breeding to develop non-toxic J. curcas genotypes. To evaluate this process, a simple and fast analytical technique was employed to obtain responses in a short time. Leaf spray (LS) is a recent ambient ionization mass spectrometry technique in which the sample itself serves as support and ion source. Here, toxic and non-toxic J. curcas leaves were differentiated by LS using a linear ion trap mass spectrometer and partial least squares discriminant analysis (PLS-DA) model chemometrics. It was possible to differentiate toxic and non-toxic leaves and to identify the m/z values that contribute to discrimination between the groups.
机译：麻风树麻风树是一种油料作物，已被研究为生物柴油的潜在来源。生产高蛋白派是生物柴油生产的副产品，可以用作动物饲料。但是，馅饼中含有有毒化合物，例如佛波酯和其他毒素，因此无法用作动物饲料。因此，巴西农业研究公司（Emrapa）一直致力于基因育种，以开发无毒的麻疯树基因型。为了评估该过程，采用了一种简单而快速的分析技术来在短时间内获得响应。叶喷雾（LS）是一种最新的环境电离质谱技术，其中样品本身充当载体和离子源。在这里，使用线性离子阱质谱仪和偏最小二乘判别分析（PLS-DA）模型化学计量学，通过LS区分有毒和无毒的麻疯树叶片。可以区分有毒和无毒叶子，并确定有助于区分各组的m / z值。

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