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首页> 外文期刊>Journal of the Brazilian Chemical Society >Chitosan/siloxane hybrid polymer: synthesis, characterization and performance as a support for immobilizing enzyme
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Chitosan/siloxane hybrid polymer: synthesis, characterization and performance as a support for immobilizing enzyme

机译：壳聚糖/硅氧烷杂化聚合物：合成，表征和作为固定化酶的载体的性能

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A hybrid polymer derived from siloxane and chitosan was obtained by sol-gel technique using tetraethoxysilane (TEOS) as a precursor. The hybrid support was chemically modified with epichlorohydrin and used to immobilize lipase from Burkholderia cepacia. The hybrid SiO2-chitosan formed new macromolecular structure in which the inorganic particles are dispersed at the nanometer scale in the organic host matrix and bounding through covalent bonds. A comparative study between free and immobilized lipase was provided in terms of pH, temperature, kinetic parameters and thermal stability. The pH for maximum hydrolysis activity shifted from 7.0 for the soluble lipase to 6.1 and the optimum temperature remained at 50 oC after immobilization. The patterns of heat stability indicated that the immobilization process provided the stabilization of the enzyme and the epoxy SiO2-chitosan derivative was almost 30-fold more stable than soluble lipase at 60 oC.
机译：使用四乙氧基硅烷（TEOS）作为前体，通过溶胶-凝胶技术获得了衍生自硅氧烷和壳聚糖的杂化聚合物。用表氯醇对杂化支持物进行化学修饰，并用于固定洋葱伯克霍尔德氏菌的脂肪酶。杂化SiO2-壳聚糖形成新的大分子结构，其中无机颗粒以纳米级分散在有机主体基质中并通过共价键结合。根据pH，温度，动力学参数和热稳定性对游离和固定化脂肪酶进行了比较研究。最大水解活性的pH从可溶性脂肪酶的7.0变为6.1，固定后的最佳温度保持在50 oC。热稳定性的模式表明，固定化过程提供了酶的稳定性，并且在60 oC下，环氧SiO2-壳聚糖衍生物的稳定性比可溶性脂肪酶高出近30倍。

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