• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Veterinary Advances >Detection of MecA, MecC and Femb Genes by Multiplex Polymerase Chain Reaction
【24h】

Detection of MecA, MecC and Femb Genes by Multiplex Polymerase Chain Reaction

机译：多重聚合酶链反应检测MecA，MecC和Femb基因

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Staphylococcus aureus causing wide range of infectious diseases affecting livestock animals as well as human beings. The bacterium is highly potent to acquire antibiotic resistance, and it is considered a very important agent inducing mastitis in dairy farms in Egypt, as well as other parts of the world, methicillin resistant S. aureus (MRSA) is deliberated one of the most significant causative agents which having public health and economic impacts especially in developing countries, so the present study aimed broadly to obtain a snapshot on the genetic characterization of mecA gene which is responsible to induce the resistance against beta lactam antibiotics. The mecA gene sequence obtained through whole genome sequence process from locally isolated S. aureus from well-established Egyptian dairy farms as well as small scale raising ones. As a control measures, the femB gene was introduced in the multiplex PCR to avoid any contaminants from other staphylococci, also, the mecC gene which is discovered recently as a homologue of mecA gene; was investigated but we did not find any isolates having this homologue.
机译：金黄色葡萄球菌引起广泛的传染病，影响到牲畜和人类。该细菌对获得抗生素耐药性非常有效，在埃及以及世界其他地区的奶牛场中，它被认为是诱发乳腺炎的非常重要的药物，耐甲氧西林金黄色葡萄球菌（MRSA）被认为是最重要的细菌之一。致病因素，特别是在发展中国家，具有公共卫生和经济影响，因此，本研究的广泛目标是获得有关mecA基因遗传特征的简要介绍，该基因负责诱导对β内酰胺类抗生素的耐药性。 mecA基因序列是通过全基因组序列过程从成熟的埃及奶牛场以及小规模饲养场的本地分离的金黄色葡萄球菌获得的。作为控制措施，将femB基因引入多重PCR以避免来自其他葡萄球菌的任何污染物，以及最近被发现为mecA基因同源物的mecC基因。已进行了调查，但没有发现具有此同源物的任何分离株。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号