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首页> 外文期刊>Journal of Venomous Animals and Toxins including Tropical Diseases >DNA identification of Leishmania (Viannia) braziliensis in human saliva from a patient with American cutaneous leishmaniasis
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DNA identification of Leishmania (Viannia) braziliensis in human saliva from a patient with American cutaneous leishmaniasis

机译：来自美国皮肤利什曼病患者的唾液中巴西利什曼原虫（Viannia）的DNA鉴定

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摘要

This study aims to report the amplification of the DNA of Leishmania (V.) braziliensis, using polymerase chain reaction, obtained from the saliva of a patient with American cutaneous leishmaniasis who did not present any lesion in the oral mucosa. Amplification produced fragments of 103 bp, an estimated size employing Leishmania (V.) braziliensis primers (b1 e b2). The present results revealed, for the first time, that the in vitro amplification of Leishmania DNA using samples from the salivary fluid of a patient with American cutaneous leishmaniasis is possible. However, more studies are required with a larger number of participants to evaluate the usefulness of saliva as a non-invasive sample for PCR. The development of such non-invasive technique is necessary for the diagnosis of many diseases in the future, especially infectious and parasitic ones.
机译：这项研究旨在报告聚合酶链反应扩增巴西利什曼原虫（V.）的DNA，该酶是从美国皮肤利什曼病患者的唾液中获得的，该患者的口腔黏膜未见任何病变。扩增产生103 bp的片段，使用巴西利什曼原虫（V.）巴西引物（b1 e b2）估计大小。本结果首次揭示，使用来自美国皮肤利什曼病病患者唾液的样品体外扩增利什曼原虫DNA是可能的。然而，需要更多的研究与更多的参与者来评估唾液作为PCR的非侵入性样品的有用性。这种无创技术的发展对于将来诊断许多疾病，尤其是传染病和寄生虫病是必要的。

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