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首页> 外文期刊>Journal of Tissue Engineering >Indirect Low-Intensity Ultrasonic Stimulation for Tissue Engineering
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Indirect Low-Intensity Ultrasonic Stimulation for Tissue Engineering

机译：用于组织工程的间接低强度超声刺激

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Low-intensity ultrasound (LIUS) treatment has been shown to increase mass transport, which could benefit tissue grafts during the immediate postimplant period, when blood supply to the implanted tissue is suboptimal. In this in vitro study, we investigated effects of LIUS stimulation on dye diffusion, proliferation, metabolism, and tropomyosin expression of muscle cells (C2C12) and on tissue viability and gene expression of human adipose tissue organoids. We found that LIUS increased dye diffusion within adjacent tissue culture wells and caused anisotropic diffusion patterns. This effect was confirmed by a hydrophone measurement resulting in acoustic pressure 150–341 Pa in wells. Cellular studies showed that LIUS significantly increased proliferation, metabolic activity, and expression of tropomyosin. Adipose tissue treated with LIUS showed significantly increased metabolic activity and the cells had similar morphology to normal unilocular adipocytes. Gene analysis showed that tumor necrosis factor-alpha expression (a marker for tissue damage) was significantly lower for stimulated organoids than for control groups. Our data suggests that LIUS could be a useful modality for improving graft survival in vivo.
机译：低强度超声（LIUS）处理已显示出可以增加质量传递，这可能会在植入后的即刻（当对植入组织的血液供应不足）时使组织移植物受益。在这项体外研究中，我们研究了LIUS刺激对肌肉细胞（C2C12）的染料扩散，增殖，代谢和原肌球蛋白表达以及对人类脂肪组织类器官组织活力和基因表达的影响。我们发现，LIUS增加了染料在邻近组织培养孔中的扩散并引起各向异性扩散模式。水听器的测量结果证实了这一影响，井中的声压为150–341 Pa。细胞研究表明，LIUS可显着增加增殖，代谢活性和原肌球蛋白的表达。用LIUS处理的脂肪组织显示出明显增加的代谢活性，并且细胞具有与正常单眼脂肪细胞相似的形态。基因分析表明，受刺激的类器官的肿瘤坏死因子-α表达（组织损伤的标志）明显低于对照组。我们的数据表明，LIUS可能是改善体内移植物存活的有用方式。

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