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首页> 外文期刊>Journal of Tissue Engineering >Application of stem-cell media to explant culture of human periosteum: An optimal approach for preparing osteogenic cell material
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Application of stem-cell media to explant culture of human periosteum: An optimal approach for preparing osteogenic cell material

机译：干细胞培养基在人骨膜外植体培养中的应用：制备成骨细胞材料的最佳方法

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As part of our clinical tests on bone regeneration using cultured periosteal sheets, here, we prepared cultured periosteal sheets in two types of stem-cell culture media, STK1 and STK3. Human periosteum was expanded either in 1% human serum–supplemented STK1 for 28 days, in 1% human serum–supplemented STK1 for 14 days followed by 1% human serum–supplemented STK3 for 14 days (1% human serum–supplemented STK1+3), or in 10% fetal bovine serum–supplemented Medium 199 for 28 days (control). Cultured periosteal sheet diameter and DNA content were significantly higher, and the multilayer structure was prominent in 1% human serum–supplemented STK1 and 1% human serum–supplemented STK1+3. The messenger RNA of osteoblastic markers was significantly upregulated in 1% human serum–supplemented STK1+3. Osteopontin-immunopositive staining and mineralization were evident across a wide area of the cultured periosteal sheet in 1% human serum–supplemented STK1+3. Subcutaneous implantation in nude mice following expansion in 1% human serum–supplemented STK1+3 produced the highest cultured periosteal sheet osteogenic activity. Expansion in 1% human serum–supplemented STK1+3 successfully induced cultured periosteal sheet growth while retaining osteogenic potential, and subsequent osteoblastic induction promoted the production of homogeneous cell material.
机译：作为我们使用培养的骨膜片进行骨再生的临床测试的一部分，在这里，我们在两种干细胞培养基STK1和STK3中准备了培养的骨膜片。人骨膜在1％的人血清中补充了STK1 28天，在1％的人血清中补充了STK1 14天，然后在1％的人血清中补充了STK3 14天（1％人的血清补充了STK1 + 3） ），或在10％胎牛血清补充的199培养基中培养28天（对照组）。培养的骨膜片的直径和DNA含量明显更高，并且在1％的人血清补充的STK1和1％的人血清补充的STK1 + 3中，多层结构突出。成骨细胞标记物的信使RNA在1％的人血清补充的STK1 + 3中显着上调。骨桥蛋白免疫阳性染色和矿化在1％人血清补充的STK1 + 3的整个培养的骨膜片中很明显。裸鼠皮下植入1％人血清补充的STK1 + 3后，可产生最高的骨膜片成骨活性。补充1％的人类血清补充的STK1 + 3可以成功诱导培养的骨膜片生长，同时保留成骨潜能，随后的成骨细胞诱导促进了均质细胞材料的产生。

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