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首页> 外文期刊>Journal of Toxicology and Environmental Health Sciences >Analyses of the effect of para- phenylenediamine Takaout roumia on the osmotic stability of human erythrocytes
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Analyses of the effect of para- phenylenediamine Takaout roumia on the osmotic stability of human erythrocytes

机译：对苯二胺高塔肉瘤对人红细胞渗透稳定性的影响分析

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摘要

This work analysed the effects of para- phenylenediamine (p-PD), a component in permanent hair dyes products on the osmotic stability of human’s erythrocytes. Hemolysis was monitored by measurement of absorbance at 412 nm following addition of erythrocytes to NaCl solutions of varying concentration. Absorbance was fitted to sigmoid regression curves given by Boltzmann equation, and hemolysis was characterized by the NaCl concentration leading to lyses of 50% of cells (H50), and by the intensity (H) and the amplitude of the lyses (dS). The parameters were determined in the absence and the presence of different doses of p-PD and at different times from 30 min to 2 h. The low doses of?p-PD used in this study (5 μg/ml) protected human erythrocytes against hypotonic shock as evidenced by a decrease of H and H50 values compared to the control solution (p<0.05) for? 2 h. However, the higher doses of?p-PD used (2.5 and 3 mg/ml) enhanced hemolysis, since their H50?values were higher than in the control group (p<0.05). In conclusion, it seems that the p-PD has simultaneously protector and haemolytic effects depending on the concentration used and the time of exposure.
机译：这项研究分析了永久性染发产品中对苯二胺（p-PD）对人红细胞渗透稳定性的影响。向不同浓度的NaCl溶液中添加红细胞后，通过测量412 nm处的吸光度来监测溶血情况。吸光度拟合由Boltzmann方程给出的S形曲线，溶血的特征是NaCl浓度导致50％细胞裂解（H50），强度（H）和裂解幅度（dS）。在不存在和存在不同剂量的p-PD的情况下以及在30分钟至2小时的不同时间确定参数。本研究中使用的低剂量的？p-PD（5μg/ ml）保护了人类红细胞免受低渗性休克的影响，这与对照溶液的H和H50值相比有所降低相吻合（p <0.05）。 2小时但是，使用更高剂量的？p-PD（2.5和3 mg / ml）会增强溶血作用，因为它们的H50？值高于对照组（p <0.05）。总之，根据所用浓度和暴露时间，p-PD似乎同时具有保护作用和溶血作用。

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