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首页> 外文期刊>Journal of Seed Science >Tetrazolium test for assessment of seed viability of atemoya (Annona cherimola Mill. x A. squamosa L.)
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Tetrazolium test for assessment of seed viability of atemoya (Annona cherimola Mill. x A. squamosa L.)

机译：四氮唑试验用于评估烟叶种子的活力（Annona cherimola Mill。x A. squamosa L.）

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摘要

The study aimed to adapt the tetrazolium test to assess the viability of atemoya (Annona cherimola Mill. x A. squamosa L.) seeds. The experimental design was completely randomized in 3 x 3 factorial arrangement (tetrazolium salt concentrations x exposure times), totaling 9 treatments with four replicates of 25 seeds. After immersion in water for 24 hours, the seeds were longitudinally sectioned and exposed to three tetrazolium salt concentrations (0.05%, 0.5% and 1%) for three periods of time (2, 4 and 6 hours). The evaluations were performed according to pre-established criteria of tissue integrity and staining. The germination test was performed, using the GA 4+7 + N-(fenilmetil)-aminopurina regulators at concentrations of 0 and 300 mg.L-1, because dormancy is observed in species of the family Annonaceae. Tetrazolium salt at 1% yielded the highest percentage of viable seeds within the shortest time, while longer exposure times were necessary with concentrations of 0.05% and 0.5%. It can be concluded that the concentration of 1% of tetrazolium salt for two hours of exposure is more appropriate for assessing the viability of atemoya seeds.
机译：该研究旨在适应四唑鎓试验以评估atemoya（Annona cherimola Mill。x A. squamosa L.）种子的生存力。实验设计完全按照3 x 3因子分解（四唑鎓盐浓度x暴露时间）完全随机分组，总共进行9次处理，其中25粒种子重复四次。浸入水中24小时后，将种子纵向切开并暴露于三种四唑盐浓度（0.05％，0.5％和1％）中三个时间段（2、4和6小时）。根据预先确定的组织完整性和染色标准进行评估。使用浓度为0和300 mg.L-1的GA 4 + 7 + N-（fenilmetil）-氨基嘌呤调节剂进行发芽试验，因为在Annonaceae科中观察到了休眠。浓度为1％的四唑盐在最短的时间内产生了最高百分比的活种子，而浓度为0.05％和0.5％的暴露时间则需要更长的时间。可以得出结论，暴露两个小时的四唑盐浓度为1％，更适合于评估atemoya种子的生存能力。

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