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首页> 外文期刊>Journal of Tissue Engineering >In vitro ovarian cancer model based on three-dimensional agarose hydrogel
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In vitro ovarian cancer model based on three-dimensional agarose hydrogel

机译：基于三维琼脂糖水凝胶的体外卵巢癌模型

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To establish a typical tumor model of ovarian cancer which may be more representative and reliable than traditional monolayer culture and pellet, agarose was used as cell vehicle to engineering tumor. Selection of agarose is based on its successful application in tissue engineering with both amenable mechanical and biological properties. In this study, ovarian cancer cell line SKOV3 was encapsulated in agarose hydrogel with cell aggregates and two-dimensional culture as controls. In vitro cell proliferation was assessed by MTT and cell viability was examined at time points of 2, 4, and 6 days. The expression of tumor malignancy markers including matrix metalloproteinase 2, matrix metalloproteinase 9, hypoxia-inducible factor-1α, and vascular endothelial growth factor–A was assessed by real-time polymerase chain reaction. The results showed that cells proliferated more rapidly in three-dimensional agarose culture than controls. Furthermore, upregulation of matrix metalloproteinase 9 and matrix metalloproteinase 2 activity and increased expression of vascular endothelial growth factor–A and hypoxia-inducible factor-1α were shown in agarose-engineered tumors. All the evidences demonstrated that agarose may provide a more favorable environment for cancer cell growth, mimicking the in vivo environment for tumor generation. The novel in vitro tumor model may be useful for the further investigation of anticancer therapeutics.
机译：为了建立一个典型的卵巢癌肿瘤模型，该模型可能比传统的单层培养和沉淀更具有代表性和可靠性，将琼脂糖用作细胞载体来工程化肿瘤。琼脂糖的选择基于其在组织工程中的成功应用，同时具有令人满意的机械和生物学特性。在这项研究中，将卵巢癌细胞系SKOV3封装在琼脂糖水凝胶中，并以细胞聚集体和二维培养作为对照。通过MTT评估体外细胞增殖，并在2、4和6天的时间点检查细胞活力。通过实时聚合酶链反应评估了包括基质金属蛋白酶2，基质金属蛋白酶9，缺氧诱导因子1α和血管内皮生长因子-A在内的肿瘤恶性标志物的表达。结果表明，在三维琼脂糖培养中，细胞的增殖比对照更快。此外，在琼脂糖工程化的肿瘤中，基质金属蛋白酶9和基质金属蛋白酶2的活性上调，血管内皮生长因子-A和缺氧诱导因子-1α的表达增加。所有证据表明，琼脂糖可能为癌细胞的生长提供了更有利的环境，模仿了体内肿瘤生成的环境。新型的体外肿瘤模型可用于进一步研究抗癌疗法。

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