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首页> 外文期刊>Journal of Veterinary Science >Mechanisms of quinolone resistance in Escherichia coli isolated from companion animals, pet-owners, and non-pet-owners
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Mechanisms of quinolone resistance in Escherichia coli isolated from companion animals, pet-owners, and non-pet-owners

机译：从伴侣动物，宠物主人和非宠物主人中分离的大肠杆菌中喹诺酮耐药机制

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The present study investigated the prevalence and mechanisms of fluoroquinolone (FQ)/quinolone (Q) resistance in Escherichia (E.) coli isolates from companion animals, pet-owners, and non-pet-owners. A total of 63 E. coli isolates were collected from 104 anal swab samples, and 27 nalidixic acid (NA)-resistant isolates were identified. Of those, 10 showed ciprofloxacin (CIP) resistance. A plasmid-mediated Q resistance gene was detected in one isolate. Increased efflux pump activity, as measured by organic solvent tolerance assay, was detected in 18 NA-resistant isolates (66.7%), but was not correlated with an increase in minimum inhibitory concentration (MIC). Target gene mutations in Q resistance-determining regions (QRDRs) were the main cause of (FQ)Q resistance in E. coli. Point mutations in QRDRs were detected in all NA-resistant isolates, and the number of mutations was strongly correlated with increased MIC (R = 0.878 for NA and 0.954 for CIP). All CIP-resistant isolates (n = 10) had double mutations in the gyrA gene, with additional mutations in parC and parE. Interestingly, (FQ)Q resistance mechanisms in isolates from companion animals were the same as those in humans. Therefore, prudent use of (FQ)Q in veterinary medicine is warranted to prevent the dissemination of (FQ)Q-resistant bacteria from animals to humans.
机译：本研究调查了来自伴侣动物，宠物主人和非宠物主人的大肠杆菌分离物中氟喹诺酮（FQ）/喹诺酮（Q）耐药的发生率和机制。从104个肛门拭子样本中总共收集了63个大肠杆菌分离株，并鉴定出27个耐萘啶酸（NA）的分离株。其中有10人显示出环丙沙星（CIP）耐药性。在一个分离物中检测到质粒介导的Q抗性基因。通过有机溶剂耐受性测定，在18种耐NA NA的菌株中检出了增加的外排泵活性（66.7％），但与最低抑菌浓度（MIC）的增加无关。 Q耐药决定区（QRDRs）中的靶基因突变是大肠杆菌（FQ）Q耐药的主要原因。在所有耐NA的菌株中均检测到QRDRs的点突变，并且突变的数量与MIC的增加密切相关（NA的R = 0.878，CIP的0.954）。所有耐CIP的分离株（n = 10）在gyrA基因中都有双重突变，在parC和parE中还有其他突变。有趣的是，从伴侣动物中分离出的（FQ）Q耐药机制与人类相同。因此，有必要在兽药中谨慎使用（FQ）Q，以防止（FQ）Q耐药细菌从动物传播到人类。

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