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首页> 外文期刊>Journal of Young Pharmacists >Isolation, biochemical and genetic characterizations of alcohol-producing yeasts from the flowers of Woodfordia fruticosa
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Isolation, biochemical and genetic characterizations of alcohol-producing yeasts from the flowers of Woodfordia fruticosa

机译：桔梗花中产酒精酵母的分离，生化和遗传特性

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Objective: To isolate and characterize the alcohol-producing yeasts from Woodfordia fruticosa flowers, which are used for the induction and maintenance of fermentation in the making of Ayurvedic formulations. Materials and methods: Initially twenty four yeasts strains were isolated on MGYP agar plate. Among them, four strains were selected for further studies on the basis of their alcohol generation capacity using jaggery media (50% w/v). Physiological, biochemical and genetic characterization (18S rRNA sequencing) of selected strains were carried out. Results: Physiological, biochemical and genetic characterization (18S rRNA sequencing) confirmed the strains as Saccharomycopsis fibuligera Jm.8, S. fibuligera Jm.10, S. fibuligera Jm.16 and Saccharomyces cerevisiae Jm.20. Under the controlled conditions, S. cerevisiae Jm.20 produced 69.57 g/l of alcohol, whereas remaining strains produced the alcohol in the range of 6.04e7.32 g/l. Conclusion: Among selected strains, strains S. fibuligera are a newer in the flowers. Kinetic study of alcohol generation revealed the strain S. cerevisiae Jm.20 can be efficiently used in making of fermented Ayurvedic formulations instead of use W. fruticosa flowers.
机译：目的：分离和鉴定金盏花（Woodfordia fruticosa）花中产酒精的酵母，这些酵母用于诱导和维持阿育吠陀制剂的发酵。材料和方法：最初在MGYP琼脂平板上分离出二十四个酵母菌株。其中，根据使用粗糖培养基（50％w / v）的酒精生成能力，选择了四种菌株进行进一步研究。进行了选定菌株的生理，生化和遗传表征（18S rRNA测序）。结果：生理，生化和遗传特征（18S rRNA测序）证实了菌株为腓肠酵母菌Jm.8，S。fibuligera Jm.10，S.fibuligera Jm.16和啤酒酵母Jm.20。在受控条件下，酿酒酵母Jm.20产生69.57 g / l的醇，而其余菌株产生6.04e7.32 g / l的醇。结论：在选定的菌株中，S。fibuligera菌株在花中是较新的。酒精生成的动力学研究表明，菌株S. cerevicere Jm.20可以有效地用于制造阿育吠陀发酵配方，而不是使用金缕梅花。

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