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首页> 外文期刊>Journal of Young Pharmacists >Quantitative determination of donepezil hydrochloride by a simple and accurate synchronous spectrofluorimetric method in human plasma
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Quantitative determination of donepezil hydrochloride by a simple and accurate synchronous spectrofluorimetric method in human plasma

机译：简单，准确的同步荧光光谱法测定人血浆中盐酸多奈哌齐

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摘要

A new simple, accurate, sensitive and specific bioanalytical spectrofluorimetric method was developed and validated for quantitative determination of donepezil hydrochloride (DH) in human plasma. Estimation of DH in human plasma was done by spiked human plasma studies. Forestimation of DH in human plasma extraction was carried out using 5% TCA forprotein precipitation followed by liquid-liquid extraction with 5% IPA in n-Hexane.Under spectrofluorimetric conditions delta value 65 applied in synchronous mode(medium sensitivity mode) and wavelength used in analysis was 389 nm. Developed method found linear, in the concentration range of 50-105 ng/mL with correlation coefficient (R2) 0.9965. Percentage recovery was found to be 75 %-85 %for DH. High recovery show that the method is free from the interference from plasma constituents. Hence proposed method can be used for estimation of DH in routine quality control laboratories for quantitative determination of DH in bulk as well as in human plasma. Further it can also be used to determine plasma DH concentration in drug monitoring or in pharmacokinetic investigations.
机译：开发了一种新的简单，准确，灵敏和特异的生物分析荧光光谱法，并已用于定量测定人血浆中盐酸多奈哌齐（DH）的有效性。通过加标人体血浆研究完成了人体血浆中DH的估计。用5％TCA沉淀蛋白质，然后用​​5％IPA在正己烷中进行液-液萃取，进行人血浆提取中DH的森林化学分析。在荧光光谱法条件下，在同步模式（中等灵敏度模式）下应用delta值65分析为389 nm。所开发的方法发现线性范围为50-105 ng / mL，相关系数（R2）为0.9965。发现DH的回收率是75％-85％。高回收率表明该方法不受血浆成分的干扰。因此，提出的方法可用于常规质量控制实验室中的DH估计，以定量测定散装以及人体血浆中的DH。此外，它还可用于在药物监测或药代动力学研究中确定血浆DH浓度。

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