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首页> 外文期刊>Journal of Young Pharmacists >High performance liquid chromatographic fluorescence detection method for the quantification of rivastigmine in rat plasma and brain: Application to preclinical pharmacokinetic studies in rats
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High performance liquid chromatographic fluorescence detection method for the quantification of rivastigmine in rat plasma and brain: Application to preclinical pharmacokinetic studies in rats

机译：高效液相色谱荧光检测法定量大鼠血浆和脑中卡巴拉汀的含量：在大鼠临床前药代动力学研究中的应用

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摘要

A highly sensitive and selective high performance liquid chromatographic fluorescence detection method has been developed and validated for the quantification of rivastigmine in rat plasma and brain. Protein precipitation and one-step liquid-liquid extraction techniques were utilized for the extraction of RSM from brain and plasma, respectively, along with an internal standard. The chromatographic separation was achieved with a column inertsil ODS-3V and a mobile phase consisting of ammonium acetate buffer (20 mM, pH 4.5) and acetonitrile (76:24, v/v) delivered at a flow rate of 1 ml/min. The lower limit of quantitation for the developed method was 10 ng/mL for both matrices. The method was found to be accurate and reproducible and was successfully used to quantify levels of RSM in plasma and brain following intravenou s administration of RSM in rats.
机译：已经开发出了一种高灵敏，选择性高的高效液相色谱荧光检测方法，并已用于定量大鼠血浆和脑中卡巴拉汀的含量。蛋白质沉淀和一步液液萃取技术分别用于从脑和血浆中提取RSM以及内标。色谱分离是通过使用inertsil ODS-3V色谱柱和由乙酸铵缓冲液（20 mM，pH 4.5）和乙腈（76:24，v / v）组成的流动相以1 ml / min的流速输送而实现的。对于两种基质，开发方法的定量下限均为10 ng / mL。发现该方法准确，可重复，已成功用于大鼠静脉内施用RSM后定量测定血浆和脑中RSM的水平。

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