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首页> 外文期刊>Journal of Young Pharmacists >Protective Effects of Anthocyanin Fraction of Berberis integerrima Bunge Fruits against H2O2 Induced Cytotoxicity in MCF7 and HepG2 Cells
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Protective Effects of Anthocyanin Fraction of Berberis integerrima Bunge Fruits against H2O2 Induced Cytotoxicity in MCF7 and HepG2 Cells

机译：小ber花色苷花色苷组分对H2O2诱导的MCF7和HepG2细胞毒性的保护作用

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Objective: Berberis integerrima Bunge show different pharmacological effects. Its fruits are rich sources of anthocyanin with biological activities. The aim of this study was evaluation of protective effects of isolated anthocyanin fraction of Berberis integerrima Bunge fruits (AFBI) against cytotoxicity induced by hydrogen peroxide (H2O2) in HepG2 and MCF-7 cell lines. Method: Cytotoxicity of ABIF and H2O2 was identified by MTT assay. In pretreatment studies, the cells were pre-exposed to nontoxic concentrations of AFBI for 24 h then cytotoxic concentration of H2O2 was added. In co-treatment study, the cells were exposed to nontoxic concentrations of AFBI and then cytotoxic concentration of H2O2, simultaneously. Result: ABIF exhibited significant toxicity at concentration of 800 μg/ml in both cell lines. The IC50 for H2O2 were 253 μM and 200 μM for MCF7 and HepG2 cells, respectively. In pre-treatment assay, anthocyanin able to increase viability of MCF7 cells at 200 and 400 μg/ml concentrations compared with the control. In HepG2 cells no significant difference in cell viability was observed compared with the control. In co-treatment study in MCF7 cells, the significant difference in cell viability was observed at all concentrations of ABIF (25-400 μg/ml), while the significant increase in cell viability were showed at 100, 200 and 400 μg/ml in HepG2 cells. Conclusion: This study suggests that AFBI is cytoprotective against H2O2-induced oxidative stress in HepG2 and MCF7. This is likely due to its antioxidant activities. This protective effect could be considered as approach for treatment of oxidative stress related diseases.
机译：目的：小Ber小show具有不同的药理作用。其果实富含花青素，具有生物活性。这项研究的目的是评估小Ber（Berberis integerrima Bunge）果实（AFBI）分离的花色苷组分对HepG2和MCF-7细胞系中过氧化氢（H2O2）诱导的细胞毒性的保护作用。方法：MTT法检测ABIF和H2O2的细胞毒性。在预处理研究中，将细胞预先暴露于无毒浓度的AFBI中24小时，然后添加细胞毒性浓度的H2O2。在共同治疗研究中，将细胞同时暴露于无毒浓度的AFBI和细胞毒性浓度的H2O2。结果：ABIF在两种细胞系中的浓度均> 800μg/ ml时均显示出明显的毒性。 H2O2的IC50对于MCF7和HepG2细胞分别为253μM和200μM。在预处理试验中，与对照相比，花色苷在200和400μg/ ml的浓度下能够增加MCF7细胞的活力。与对照相比，在HepG2细胞中未观察到细胞活力的显着差异。在MCF7细胞的协同治疗研究中，在所有ABIF（25-400μg/ ml）浓度下均观察到细胞活力的显着差异，而在100、200和400μg/ ml的条件下，细胞活力显着增加。 HepG2细胞。结论：这项研究表明AFBI对H2O2诱导的HepG2和MCF7氧化应激具有细胞保护作用。这可能是由于其抗氧化活性。这种保护作用可以被认为是治疗氧化应激相关疾病的方法。

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