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首页> 外文期刊>Journal of Young Pharmacists >Quantification of Tamoxifen Polymeric Nanoparticles in female rodent breast tissue by UPLC/ESI-Q-TOF MS/MS
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Quantification of Tamoxifen Polymeric Nanoparticles in female rodent breast tissue by UPLC/ESI-Q-TOF MS/MS

机译：通过UPLC / ESI-Q-TOF MS / MS对雌性啮齿动物乳腺组织中他莫昔芬聚合物纳米颗粒的定量

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Objective: The present work aims to develop and validate stability indicating a novel liquid chromatography method with applicability i.e. to study the pharmacokinetics factor as well as estimate Tamoxifen (TMX) in bio-analytes, using Ultra High Pressure Liquid Chromatography/ Mass Spectrometry (UPLC-ESI-Q-TOF-MS/MS) in plasma. Methods: A bioanalytical method based on UPLC-ESI-Q-TOF-MS/MS has been developed and validated for the quantitative determination of TMX in female mice plasma using Clomiphine as an Internal Standard (IS). After Liquid-liquid extraction (LLE), analyte and IS, chromatographic separation was achieved on ACQUITY UPLC BEH C18 Waters column with dimensions; 100 mm × 2.1 mm; 1.7 μm, isocratic mobile phase (Acetonitrile:2 mM ammonium formate: 90:10; v/v), and a flow rate of 0.25 mL min-1. Results: The transitions occurred at m/z 372.1→178.1 and m/z 407.1 → 100.1 for TMX and IS, respectively. Liquidliquid extraction technique (LLE) using ethyl acetate was applied in order to optimize the recovery of analytes in mice plasma. The run and retention time of TMX were 6.0 and 2.63 minutes, respectively while the linear dynamic range established was 1.002-4001.07 ng/ml (r2>0.998 ± 0.0003). Intra-assay and inter-assay accuracy (% RSD) was found in the range; 2.43- 3.49. Conclusion: The proposed LC–MS/MS assay method is simple, rapid and sensitive for the determination of TMX in mice plasma for pharmacokinetic studies. Analytes were stable under various conditions i.e. autosampler, freeze–thaw, at room temperature, and under deep freeze conditions.
机译：目的：目前的工作旨在开发和验证稳定性，从而表明一种适用的新型液相色谱方法，即使用超高压液相色谱/质谱法（UPLC-R）研究生物分析物中的药代动力学因子并估算他莫昔芬（TMX）血浆中的ESI-Q-TOF-MS / MS）。方法：已开发出一种基于UPLC-ESI-Q-TOF-MS / MS的生物分析方法，并验证了使用氯米芬作为内标（IS）定量测定雌性小鼠血浆中TMX的方法。进行液-液萃取（LLE），分析物和IS后，在ACQUITY UPLC BEH C18 Waters色谱柱上完成了色谱分离; 100毫米×2.1毫米; 1.7μm，等度流动相（乙腈：2 mM甲酸铵：90：10; v / v），流速为0.25 mL min -1 。结果：TMX和IS的转变分别发生在m / z 372.1→178.1和m / z 407.1→100.1。为了优化小鼠血浆中分析物的回收率，应用了使用乙酸乙酯的液液萃取技术（LLE）。 TMX的运行时间和保留时间分别为6.0和2.63分钟，而建立的线性动态范围为1.002-4001.07 ng / ml（r2> 0.998±0.0003）。测定内和测定间准确性（％RSD）在该范围内; 2.43-3.49。结论：拟议的LC-MS / MS测定方法简便，快速，灵敏，可用于测定药代动力学研究的小鼠血浆中的TMX。在各种条件下，即自动进样器，室温下的冻融和深度冷冻条件下，分析物都稳定。

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