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首页> 外文期刊>Journal of Young Pharmacists >Gene Expression Levels of Selected Factors in Monocytic Leukemia Cell Line THP-1 Upon Treatment with n-butanol Extract of Atractylis flava Desf against Cancer
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Gene Expression Levels of Selected Factors in Monocytic Leukemia Cell Line THP-1 Upon Treatment with n-butanol Extract of Atractylis flava Desf against Cancer

机译：用白术提取物对癌症的单核细胞白血病细胞株THP-1中所选因子的基因表达水平

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Objective: The aim of the present study was to screen the anticancer activity of Atractylis flava Desf in butanolic extract (AFBE). In addition, we attempted to investigate the gene expression levels of seven relevant genes involved in the pathways leading to toxicity: oxidative stress (NCF1, OPA1, SDHA), inflammation (TNFα), apoptotic balance (PDCD4, BCL2, CASP8) and the measurement of caspase-3 activity on the acute monocytic leukemia cell line THP-1. Material and Methods: The cell viability was assessed using Trypan blue exclusion, alamarBlue? and WST-1 assays. The gene expression levels were tested by RT-qPCR. Activity of caspase-3 activity was performed using the EnzChek? Caspase-3 Assay kit to confirm the induction of apoptosis on tumor cells. Results: We observed significant growth inhibitory activity of the AFBE extract on the acute monocytic leukemia cell line THP-1. Moreover, the gene expression analysis showed that the plant extract caused statistically significant downregulation of selected genes compared to the untreated cells. Caspase-3 activity in the treated cells was significantly elevated. Conclusion: The presents study states that the butanolic extract of Atractylis flava Desf showed a significant cytotoxic effect against THP-1 cells. Our results suggest that the AFBE may be beneficial for the treatment and prevention of cancer.
机译：目的：本研究的目的是筛选丁香提取物（AFBE）中白术的抗癌活性。此外，我们试图研究涉及导致毒性的途径的七个相关基因的基因表达水平：氧化应激（NCF1，OPA1，SDHA），炎症（TNFα），细胞凋亡平衡（PDCD4，BCL2，CASP8）和测量方法。蛋白酶3活性对急性单核细胞白血病细胞THP-1的影响材料和方法：使用台盼蓝排除法alamarBlue？评估细胞活力。和WST-1分析。通过RT-qPCR测试基因表达水平。使用EnzChek？进行caspase-3活性的活性。 Caspase-3检测试剂盒，用于证实诱导肿瘤细胞凋亡。结果：我们观察到AFBE提取物对急性单核细胞白血病细胞THP-1具有明显的生长抑制活性。此外，基因表达分析表明，与未处理的细胞相比，植物提取物在统计学上显着下调了所选基因的下调。经处理的细胞中的Caspase-3活性显着提高。结论：目前的研究表明，白术的丁醇提取物对THP-1细胞具有明显的细胞毒性作用。我们的结果表明，AFBE可能有益于癌症的治疗和预防。

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