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首页> 外文期刊>Journal of Translational Medicine >Histamine release and fibrinogen adsorption mediate acute inflammatory responses to biomaterial implants in humans
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Histamine release and fibrinogen adsorption mediate acute inflammatory responses to biomaterial implants in humans

机译：组胺的释放和纤维蛋白原的吸附介导了人类对生物材料植入物的急性炎症反应

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Background Medical implants often fail as a result of so-called foreign body reactions during which inflammatory cells are recruited to implant surfaces. Despite the clinical importance of this phenomenon, the mechanisms involved in these reactions to biomedical implants in humans are not well understood. The results from animal studies suggest that both fibrinogen adsorption to the implant surface and histamine release by local mast cells are involved in biomaterial-mediated acute inflammatory responses. The purpose of this study was to test this hypothesis in humans. Methods Thirteen male medical student volunteers (Caucasian, 21–30 years of age) were employed for this study. To assess the importance of fibrinogen adsorption, six volunteers were implanted with polyethylene teraphthalate disks pre-coated with their own (fibrinogen-containing) plasma or (fibrinogen-free) serum. To evaluate the importance of histamine, seven volunteers were implanted with uncoated disks with or without prior oral administration of histamine receptor antagonists. The acute inflammatory response was estimated 24 hours later by measuring the activities of implant-associated phagocyte-specific enzymes. Results Plasma coated implants accumulated significantly more phagocytes than did serum coated implants and the recruited cells were predominantly macrophage/monocytes. Administration of both H1 and H2 histamine receptor antagonists greatly reduced the recruitment of macrophages/monocytes and neutrophils on implant surfaces. Conclusion In humans – as in rodents – biomaterial-mediated inflammatory responses involve at least two crucial events: histamine-mediated phagocyte recruitment and phagocyte accumulation on implant surfaces engendered by spontaneously adsorbed host fibrinogen. Based on these results, we conclude that reducing fibrinogen:surface interactions should enhance biocompatibility and that administration of histamine receptor antagonists prior to, and shortly after, medical device implantation should improve the functionality and longevity of medical implants.
机译：背景技术医用植入物经常由于所谓的异物反应而失败，在异物反应期间，炎症细胞被募集到植入物表面。尽管该现象在临床上具有重要意义，但人们对这些对生物医学植入物的反应所涉及的机制尚不十分清楚。动物研究的结果表明，纤维蛋白原吸附到植入物表面和局部肥大细胞释放组胺都与生物材料介导的急性炎症反应有关。这项研究的目的是检验人类的这一假设。方法采用13名男性医学生志愿者（白种人，21-30岁）进行这项研究。为了评估纤维蛋白原吸附的重要性，六名志愿者植入了聚对苯二甲酸乙二醇酯圆盘，这些圆盘预先涂有自己的（含纤维蛋白原）血浆或（不含纤维蛋白原）血清。为了评估组胺的重要性，七名志愿者在未口服或未口服组胺受体拮抗剂的情况下植入了未包被的椎间盘。 24小时后通过测量与植入物相关的吞噬细胞特异性酶的活性来估计急性炎症反应。结果血浆涂覆的植入物比血清涂覆的植入物积聚了更多的吞噬细胞，募集的细胞主要是巨噬细胞/单核细胞。同时使用H1和H2组胺受体拮抗剂可大大减少巨噬细胞/单核细胞和嗜中性粒细胞在植入物表面的募集。结论在人类中-与在啮齿动物中一样-生物材料介导的炎症反应至少涉及两个关键事件：组胺介导的吞噬细胞募集和吞噬细胞因自发吸附的宿主纤维蛋白原而引起的植入物表面积聚。基于这些结果，我们得出结论，减少纤维蛋白原：表面相互作用应增强生物相容性，并且在医疗器械植入之前和之后不久施用组胺受体拮抗剂应可以改善医疗植入物的功能和寿命。

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