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首页> 外文期刊>Journal of Translational Medicine >MicroRNA-142-5p contributes to Hashimoto’s thyroiditis by targeting CLDN1
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MicroRNA-142-5p contributes to Hashimoto’s thyroiditis by targeting CLDN1

机译：MicroRNA-142-5p通过靶向CLDN1促进桥本甲状腺炎

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Background MicroRNAs have the potential as diagnostic biomarkers and therapeutic targets in autoimmune diseases. However, very limited studies have evaluated the expression of microRNA profile in thyroid gland related to Hashimoto’s thyroiditis (HT). Methods MicroRNA microarray expression profiling was performed and validated by quantitative RT-PCR. The expression pattern of miR-142-5p was detected using locked nucleic acid-in situ hybridization. The target gene was predicted and validated using miRNA targets prediction database, gene expression analysis, quantitative RT-PCR, western blot, and luciferase assay. The potential mechanisms of miR-142-5p were studied using immunohistochemistry, immunofluorescence, and quantitative assay of thyrocyte permeability. Results Thirty-nine microRNAs were differentially expressed in HT (Fold change?≥2, P?Conclusions Our findings indicate a previously unrecognized mechanism that miR-142-5p, targeting CLDN1, plays an important role in HT pathogenesis.
机译：背景技术MicroRNA在自身免疫性疾病中具有作为诊断生物标志物和治疗靶标的潜力。然而，非常有限的研究评估了与桥本氏甲状腺炎（HT）相关的甲状腺中microRNA谱的表达。方法进行MicroRNA微阵列表达谱分析，并通过定量RT-PCR验证。使用锁定的核酸原位杂交检测miR-142-5p的表达模式。使用miRNA靶标预测数据库，基因表达分析，定量RT-PCR，蛋白质印迹和荧光素酶测定法对靶标基因进行了预测和验证。使用免疫组织化学，免疫荧光和甲状腺细胞通透性定量分析研究了miR-142-5p的潜在机制。结果39个microRNA在HT中差异表达（倍数变化≥2，P？结论）我们的发现表明，以前未知的机制是靶向CLDN1的miR-142-5p在HT发病机理中起重要作用。

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