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首页> 外文期刊>Jundishapur Journal of Microbiology >Genetic Determinants of Resistance to Fusidic Acid Among Staphylococcus aureus Isolates in Jordan
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Genetic Determinants of Resistance to Fusidic Acid Among Staphylococcus aureus Isolates in Jordan

机译：约旦州金黄色葡萄球菌分离株对夫斯地酸的遗传决定因素

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Fusidic acid-resistant Staphylococcus aureus (FRSA) has been reported in many countries to have a remarkable difference in resistance determinants. Fusidic acid resistance is very important because it might lead to the failure of topical treatment, especially when it is used as empiric therapy. In addition, its resistance might be linked to other antibiotic resistances. The overall rate of fusidic acid resistance is still relatively low. However, there is an increase in the prevalence of clinical isolates of FRSA worldwide. We aimed to characterize FRSA isolated from Jordanian patients and evaluate the occurrence of the genetic resistance caused by fusB and fusC.We conducted a prospective cross-sectional study to determine the prevalence and the resistance pattern of S. aureus to fusidic acid among Jordanian patients and healthy people. Staphylococcus aureus clinical isolates (n = 113) obtained from patients admitted to Prince Hamzah Hospital between February and July 2015 were compared with isolates (n = 288) obtained from healthy subjects. Conventional methods were used for the identification of S. aureus and further confirmations were done by the existence of the thermonuclease gene using polymerase chain reaction (PCR). Screenings of antibiotic resistance were performed using the disc diffusion method. The minimum inhibitory concentrations were calculated using the E-test. PCR was used to detect the presence of resistant genes.The FRSA frequency was significantly higher among clinical isolates (31.9%) than among isolates from healthy subjects (1%) and in methicillin-resistant Staphylococcus aureus (MRSA) (66.7%) than in methicillin-sensitive Staphylococcus aureus (MSSA) (33.3%). Of the FRSA isolates, 38.9% and 16.7% carried fusB and fusC, respectively, and they displayed low resistance compared to non-fusB, non-fusC FRSA isolates. The rate of FRSA was significantly (P & 0.05) higher among MRSA than among MSSA isolates (n = 24, 66.7% and n = 12, 33.3%, respectively). We found no association between fusidic acid determinants among MRSA and MSSA (P & 0.05).A high occurrence of FRSA was detected in Jordanian clinical isolates of S. aureus, particularly among MRSA. Moreover, fusB was the predominant resistance determinant, with low-level resistance. Based on our findings, fusidic acid susceptibility testing is strongly recommended in medical laboratories. The restricted use of fusidic acid is advised.
机译：在许多国家，据报道对夫西地酸具有抗药性的金黄色葡萄球菌（FRSA）在抗药性决定因素上有显着差异。夫西地酸的耐药性非常重要，因为它可能导致局部治疗失败，尤其是在用作经验治疗时。此外，其耐药性可能与其他抗生素耐药性有关。夫西地酸的总体耐药率仍然较低。但是，全世界FRSA的临床分离株患病率增加。我们旨在表征分离自约旦患者的FRSA并评估由fusB和fusC引起的遗传抗性的发生率。我们进行了一项前瞻性横断面研究，以确定约旦患者和患者中金黄色葡萄球菌对夫西地酸的患病率和抗性模式健康的人。将2015年2月至7月间从Hamzah王子医院住院的患者获得的金黄色葡萄球菌临床分离株（n = 113）与从健康受试者获得的分离株（n = 288）进行了比较。使用常规方法鉴定金黄色葡萄球菌，并使用聚合酶链反应（PCR）通过热核酸酶基因的存在进一步确认。使用圆盘扩散法进行抗生素耐药性筛查。最小抑菌浓度使用E检验计算。 PCR检测抗药性基因的存在。临床分离株（31.9％）的FRSA频率显着高于健康受试者分离株（1％）和耐甲氧西林金黄色葡萄球菌（MRSA）的FRSA频率（66.7％）。耐甲氧西林的金黄色葡萄球菌（MSSA）（33.3％）。在FRSA分离物中，分别携带fusB和fusC的占38.9％和16.7％，与非FusB，非FusC FRSA分离物相比，它们显示出较低的抗性。在MRSA中，FRSA的比率显着（P <0.05）比MSSA分离株中的高（分别为n = 24、66.7％和n = 12、33.3％）。我们发现在MRSA和MSSA之间的夫西地酸决定因素之间没有关联（P> 0.05）。在约旦的金黄色葡萄球菌临床分离株中，特别是在MRSA中，检测到FRSA的发生率很高。而且，fusB是主要的电阻决定因素，具有低水平的电阻。根据我们的发现，强烈建议在医学实验室进行夫西地酸敏感性测试。建议限制使用夫西地酸。

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