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首页> 外文期刊>Jundishapur Journal of Microbiology >SCCmec and spa Typing of Staphylococcus aureus Strains Isolated from Patients with Urinary Tract Infection: Emergence of spa Types t426 and t021 in Iran
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SCCmec and spa Typing of Staphylococcus aureus Strains Isolated from Patients with Urinary Tract Infection: Emergence of spa Types t426 and t021 in Iran

机译：从尿路感染患者中分离出的金黄色葡萄球菌菌株的SCCmec和spa分型：伊朗t426和t021型spa的出现

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Urinary tract infection (UTI) is one of the most frequent types of infections in community and hospital settings.The aim of the present study was to investigate antimicrobial susceptibility patterns and also to determine spa and SCCmec types of Staphylococcus aureus strains isolated from patients with UTI.During a 12-month period, 863 urine samples were investigated. In vitro susceptibility of isolates was performed using the Kirby-Bauer disk diffusion method. Moreover, conventional PCR was performed to detect mecA, nucA, spa, and toxin (pvl, tst) genes as well as Multiplex PCR to determine SCCmec types.A total of 90 isolates (10.4%) were analyzed in the present survey. Methicillin resistance was observed in 61.1% of the isolates. In total, 25 isolates (27.8%) were positive for the pvl-encoding gene and 40 (44.4%) for tst-1 encoding gene. Based on a multiplex PCR assay, the 4 different SCCmec types were detected as type III (38.9%), followed by type II (31.1%), type IV (28.9%), and type I (1.1%). Panton-Valentine Leukocidin (PVL) positive isolates belonged to SCCmec type IV (14.4%) and II (13.3%). The 90 S. aureus isolates were classified to 10 spa types t037 (23.3%), t924 (15.6%), t383 (15.6%), t426 (12.2%), t044 (12.2%), t790 (6.7%), t084 (4.4%), t021 (4.4%), t7580 (4.4%), and t064 (1.2%). The spa types t044, t790, and t084 were obtained from methicillin-resistant S. aureus (MRSA) strains and spa types t924 and t7580 from methicillin-sensitive S. aureus strains.To the best of the authora??s knowledge, this is the first study on spa types t426 and t021 in Iran. Since the majority of strains isolated from patients with UTI were MRSA, detection of MRSA clones and their characteristics is necessary.
机译：尿路感染（UTI）是社区和医院环境中最常见的感染类型之一。本研究的目的是调查抗菌药的敏感性模式，并确定从UTI患者中分离出的金黄色葡萄球菌菌株的spa和SCCmec类型在12个月内，对863个尿液样本进行了调查。使用Kirby-Bauer圆盘扩散法进行分离株的体外药敏试验。此外，进行常规PCR检测mecA，nucA，spa和毒素（pvl，tst）基因，以及进行多重PCR以确定SCCmec类型。本次调查共分析了90种分离株（10.4％）。在61.1％的分离物中观察到了对甲氧西林的耐药性。总共有25个分离株（27.8％）对pvl编码基因呈阳性，对tst-1编码基因呈阳性40个（44.4％）。根据多重PCR分析，检测到4种不同的SCCmec类型为III型（38.9％），其次是II型（31.1％），IV型（28.9％）和I型（1.1％）。 Panton-Valentine Leukocidin（PVL）阳性分离株分别属于IV型SCCmec（14.4％）和II型SCCmec（13.3％）。将90株金黄色葡萄球菌分为10种水疗类型t037（23.3％），t924（15.6％），t383（15.6％），t426（12.2％），t044（12.2％），t790（6.7％），t084（ 4.4％），t021（4.4％），t7580（4.4％）和t064（1.2％）。从耐甲氧西林的金黄色葡萄球菌（MRSA）菌株获得水疗类型t044，t790和t084，从对甲氧西林敏感的金黄色葡萄球菌菌株获得水疗类型t924和t7580。伊朗对t426和t021水疗中心类型的首次研究。由于从UTI患者中分离出的大多数菌株均为MRSA，因此必须检测MRSA克隆及其特征。

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