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首页> 外文期刊>Journal of Medical Microbiology: An Official Journal of the Pathological Society of Great Britain and Ireland >Comparison of two matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry methods and API 20AN for identification of clinically relevant anaerobic bacteria
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Comparison of two matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry methods and API 20AN for identification of clinically relevant anaerobic bacteria

机译：比较两种基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱方法和API 20AN来鉴定临床上相关的厌氧细菌

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摘要

Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is suitable for high-throughput and rapid microbial diagnosis at relatively low cost and can be considered an alternative to conventional biochemical and molecular identification systems in clinical microbiological laboratories, including anaerobe laboratories. Two commercially available MALDI-TOF MS systems, Bruker Microflex MS and bioMérieux VITEK MS, were evaluated for the identification of 274 isolates of clinically significant anaerobic bacteria recovered from routine cultures of clinical specimens in parallel with blinded comparison with conventional biochemical (API 20AN) or molecular methods. All were recovered cultures obtained from patients attending the Mubarak Al Kabir Hospital, Kuwait, during a 6 month period. Discrepant results after two attempts at direct colony testing had failed to provide acceptable MALDI-TOF identification were resolved by gold-standard 16S gene sequencing. The VITEK MS gave high-confidence identification of the 274 isolates, all of which were correctly identified. The Bruker Microflex MS system also gave high-confidence identification for 272 of the 274. After discrepancy testing, the Bruker MS results agreed with biochemical or molecular methods for 89.1 % of the isolates at species level and 10.2 % at genus level (0.72 % were misidentified). The level of agreement with the VITEK MS was 100 % at both species and genus level; no isolates were misidentified. Our data suggest that implementation of MALDI-TOF MS as a first step for identification will shorten the turnaround time and reduce the cost in the anaerobe laboratory.
机译：基质辅助激光解吸/电离飞行时间质谱仪（MALDI-TOF MS）适用于以相对较低的成本进行高通量和快速微生物诊断，并且可以认为是临床微生物实验室中常规生化和分子鉴定系统的替代品，包括厌氧菌实验室。评估了两种市售的MALDI-TOF MS系统，即Bruker Microflex MS和bioMérieuxVITEK MS，以鉴定从临床标本常规培养物中回收的274种临床上重要的厌氧菌，同时与常规生化试剂（API 20AN）进行盲法比较分子方法。所有这些都是在六个月内从科威特Mubarak Al Kabir医院就诊的患者那里获得的培养物。两次直接菌落试验均未能提供可接受的MALDI-TOF鉴定结果，结果不一致，已通过金标准16S基因测序解决。 VITEK MS对274个分离株进行了高可信度鉴定，所有分离物均已正确鉴定。 Bruker Microflex MS系统还对274个中的272个进行了高可信度鉴定。差异测试后，Bruker MS结果与生物化学或分子方法相符，在物种水平上为89.1％，在属水平上为10.2％（0.72％错误识别）。在物种和属水平上，与VITEK MS的一致水平均为100％;没有发现任何分离物。我们的数据表明，将MALDI-TOF MS用作识别的第一步将缩短周转时间并降低厌氧菌实验室的成本。

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