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首页> 外文期刊>BMC Genomics >Comparative analysis of somitogenesis related genes of the hairy/Enhancer of split class in Fugu and zebrafish
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Comparative analysis of somitogenesis related genes of the hairy/Enhancer of split class in Fugu and zebrafish

机译：府谷和斑马鱼毛状/分裂类增强子的体发生相关基因的比较分析

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Background Members of a class of bHLH transcription factors, namely the hairy (h), Enhancer of split (E(spl)) and hairy-related with YRPW motif (hey) (h/E(spl)/hey) genes are involved in vertebrate somitogenesis and some of them show cycling expression. By sequence comparison, identified orthologues of cycling somitogenesis genes from higher vertebrates do not show an appropriate expression pattern in zebrafish. The zebrafish genomic sequence is not available yet but the genome of Fugu rubripes was recently published. To allow comparative analysis, the currently known Her proteins from zebrafish were used to screen the genomic sequence database of Fugu rubripes. Results 20 h/E(spl)/hey-related genes were identified in Fugu, which is twice the number of corresponding zebrafish genes known so far. A novel class of c-Hairy proteins was identified in the genomes of Fugu and Tetraodon. A screen of the human genome database with the Fugu proteins yielded 10 h/E(spl)/hey-related genes. By analysing the upstream sequences of the c-hairy class genes in zebrafish, Fugu and Tetraodon highly similar sequence stretches were identified that harbour Suppressor of hairless paired binding sites (SPS). This motif was also discovered in the upstream sequences of the her1 gene in the examined fish species. Here, the Su(h) sites are separated by longer intervening sequences. Conclusions Our study indicates that not all her homologues in zebrafish have been isolated. Comparison to the human genome suggests a selective duplication of h/E(spl) genes in pufferfish or loss of members of these genes during evolution to the human lineage.
机译：一类bHLH转录因子的背景成员，包括毛发（h），分裂增强子（E（spl））和与YRPW主题相关的毛发相关（hey）（h / E（spl）/ hey）基因脊椎动物的体发生，其中一些表现出循环表达。通过序列比较，已鉴定的来自高级脊椎动物的循环体发生基因的直向同源物在斑马鱼中未显示适当的表达模式。斑马鱼的基因组序列尚不可用，但是最近发表了古河豚的基因组。为了进行比较分析，使用了目前已知的来自斑马鱼的Her蛋白来筛选河豚鱼的基因组序列数据库。结果在Fugu中鉴定出20 h / E（spl）/ hey相关基因，是迄今为止已知的相应斑马鱼基因数量的两倍。在Fugu和Tetraodon的基因组中鉴定出一类新型的c-Hairy蛋白。带有Fugu蛋白的人类基因组数据库的筛选产生了10 h / E（spl）/ hey相关基因。通过分析斑马鱼中c-hairy类基因的上游序列，Fugu和Tetraodon高度相似的序列被鉴定为具有无毛配对结合位点（SPS）的抑制剂。在被检鱼类中her1基因的上游序列中也发现了该基序。在这里，Su（h）位点由更长的插入序列隔开。结论我们的研究表明，并非斑马鱼中的所有同系物都已被分离出来。与人类基因组的比较表明河豚中h / E（spl）基因的选择性复制或在进化到人类谱系期间这些基因成员的丢失。

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